Characterisation of kinetic parameters and metabolic transition of Corynebacterium glutamicum on L-lysine production in continuous culture

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Title
Characterisation of kinetic parameters and metabolic transition of Corynebacterium glutamicum on L-lysine production in continuous culture
Author(s)
Hong Weon Lee; Jae Gu Pan; J M Lebeault
Bibliographic Citation
Applied Microbiology and Biotechnology, vol. 43, no. 6, pp. 1019-1027
Publication Year
1995
Abstract
Kinetic parameters and physiological states of Corynebacterium glutamicum at the growing and L-lysine-overproducing phase were characterised in continuous culture on threonine-limited complex and minimal media. High L-lysine productivity occurred at dilution rates ranging from 0.1 h-1 to 0.3 h-1 on threonine-limited complex medium, and at dilution rates ranging from 0.1 h-1 to 0.15 h-1 in minimal medium. L-Lysine yields of 0.25 g/g (0.31 gig as L-lysine hydrochloride) in complex medium, and of 0.17 g/g (0.21 g/g as L-lysine hydrochloride) in minimal medium, corresponding respectively to intrinsic yields of 0.533 g/g and 0.572 g/g were obtained. These intrinsic yield factors are closed to the theoretical ones (0.608 g/g, 0.75 mol/mol). Intrinsic biomass yields were calculated as 0.658 g/g in complex medium and 0.283 g/g in minimal medium. CO2 production has been clearly related to L-lysine production. According to our results on specific uptake rates and specific productivities in complex medium, metabolic rearrangement should occur during the transition from the growing phase to the L-lysine-overproducing phase. This phenomenon was further investigated.
Keyword
lysinebacterial metabolismbacterium culturecontrolled studycorynebacterium glutamicumkinetics
ISSN
0175-7598
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00166919
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > 1. Journal Articles
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