Brevibacterium ketoglutamicum을 이용한 L-ornithine 생산 연구 part 1 : L-ornithine 생산 고역가 안정화 균주 선발

Abstract

Over production of L-ornithine by mutant strains isolated from Brevibacterium ketoglutamicum BK1046 was investigated. The strain was a L- citrulline auxotroph and exhibited culture instability during fermentation. Through a sequential screening effort, a highly stable strain with less revertant formation was finally selected and designated B. ketoglutamicum BK52 (KCTC 0141BP). It produced L-ornithine at a high concentration (above 9 g/L) independent of subculture or cultivation time, and also had a very low tendency of revertant formation. In a long-term storage, this strain maintained its cell stability and productivity of L-ornithine to a reasonable range.