Brevibacterium ketoglutamicum을 이용한 L-ornithine 생산 연구 part II : L-arginine 제한공급에 의한 L-ornithine 유가식 발효생산

Abstract

A highly productive fed-batch fermentation process was developed for the production of L-ornithine by using a new stabilized strain, Brevibacterium ketoglutamicum BK52. Fed-batch cultures with a continuous feeding of the complex medium were conducted on various operating conditions. The optimal concentration of phosphate in the complex medium was 2.1 g/L. The optimal feeding rate of L-arginine was 0.028 g/L/hr. The optimal feeding point of the complex medium was determined to be at 40 OD of the cell mass. The final L- ornithine concentrations within 64 hrs of cultivation in 5 and 50 liter fermenters were 73 g/L and 71 g/L, respectively. The maximum overall L- ornithine productivity was 1.14 g/L/hr which was about 2 times higher than that of the conventional fed-batch culture with intermittent feeding. The overall productivity of the fermentation system is remarkably improved by employing the optimized conditions, and it offers a significant potential for industrial application.