Effects of mixing on fed-batch fermentation of L-ornithine

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Title
Effects of mixing on fed-batch fermentation of L-ornithine
Author(s)
Hong Weon Lee; Sun Jun Yoon; Hyung Wook Jang; Chun Sug Kim; Tae Hyo Kim; Wuk Sang Ryu; Joon Ki Jung; Young Hoon Park
Bibliographic Citation
Journal of Bioscience and Bioengineering, vol. 89, no. 6, pp. 539-544
Publication Year
2000
Abstract
The effects of mixing on L-ornithine fermentation were studied using an L-arginine auxotrophic mutant of Brevibacterium ketoglutamicum ATCC 21092. Three different modes of fed-batch culture using two different impeller types were studied in a 7 l fermentor. The first two modes of fed-batch culture were carried out in a fermentor equipped with six-flat-blade disk-turbine impellers with top and bottom feeding. The third mode of fed batch culture was carried out using pitched-six-flat-blade disk-turbine impellers with top feeding. The titre of L-ornithine increased up to 1.8 fold with bottom- feeding or when the pitched-six-flat-blade disk-turbine impellers were used compared to when the six-flat-blade disk-turbine impellers with top-feeding were used. It was observed that the mixing time of the limiting nutrients varied significantly depending on both the direction of feeding and the impeller type. Since the profiles of the specific rates of CO2 evolution, oxygen and glucose uptakes were very similar for the three culture modes, it could be reasonably assumed that the microorganism exhibited similar growth rates for each mode used. However, different amino acid producing activities were observed in the three culture modes. From these results it is concluded that the productivity of L-ornithine fermentation is significantly improved by shortening the mixing time of the limiting nutrient in the fermentor.
Keyword
L-ornithinefed-batch fermentationmixing effectscale-up
ISSN
1389-1723
Publisher
Soc Bioscience Bioengineering Japan
DOI
http://dx.doi.org/10.1016/S1389-1723(00)80053-5
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > 1. Journal Articles
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