Cytotoxicity of shikonin metabolites with biotransformation of human intestinal bacteria

Cited 0 time in scopus
Metadata Downloads
Cytotoxicity of shikonin metabolites with biotransformation of human intestinal bacteria
Byung Sun Min; Meselhy R Meselhy; Masao Hattori; Hwan Mook Kim; Young Ho Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 10, no. 4, pp. 514-517
Publication Year
Six shikonin metabolites were obtained from human intestinal bacteria, Bacteriodes fragilis subsp. thetaotus, following biotransformation. The transformation of shikonin (1) was performed anaerobically for 3 day at 37°C in the bacterial suspension of B. fragilis which was cultured overnight in GAM broth. The incubation mixture was extracted with EtOAc to give a dark-brown residue. The residue was applied to a Silica gel column, which was eluted successively with hexane (Fr. A), CHCl3 (Fr. B), and CHCl3:MeOH (9:1) (Fr. C). Six metabolites, Fr.A (2 and 3), Fr. B (6 and 7), and Fr. C (4 and 5) were isolated by repeated silica gel column chromatography, preparative TLC, followed by Sephadex LH-20. In vitro cytotoxicities were tested against human tumor cell lines; PC-3 (prostate), ACHN (renal), A549 (lung), SW620 (colon), K562 (leukemia), and Du 145 (prostate). The Shikonin metabolites 2, 4, 5, and 6 showed weaker cytotoxicity than the parent shikonin (1), whereas shikonin monomeric metabolite 3 (ED50 0.44- 1.22 μg/ml) and dimeric metabolite 7 (ED50 0.48-2.35 μg/ml) exhibited stronger activities compared with adriamycin, which was used as the positive control.
ShikoninbiotransformationBacteriodes fragilis subsp. thetaotuscytotoxicity
Korea Soc-Assoc-Inst
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.