Analysis of the genome of Symbiobaterium toebii by pulsed-field gel electrophoresis

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Title
Analysis of the genome of Symbiobaterium toebii by pulsed-field gel electrophoresis
Author(s)
Seung Pyo Hong; Jong Hoon Park; Yong Sung Kim; Hae Jun Hwang; Sung-Keun Rhee; Seung Goo Lee; Moon Hee Sung; Nobuyoshi Esaki
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 10, no. 3, pp. 405-409
Publication Year
2000
Abstract
We have studied the genome of an obligately commensal thermophile, Symbiobacterium toebii. The chromosome was extracted from pure cultures of S. toebii recently established. Total DNA of S. toebii was resolved by pulsed- field gel electrophoresis (PFGE) into discrete numbers of fragments by digestion with the endonuclease SspI, SpeI, XbaI, and HpaI. Estimated sizes of fragments produced by the four enzymes and their sum consistently yielded a total genome size of 2.8 Mb. Because restriction endonucleases NotI and SwaI, recognizing 8 bp, released too many fragments, these enzymes could not be used for the estimation of the genome size. Considering no mobility of undigested genome under PFGE, the genome of S. toebii appears to be circular. The presence of extrachromosomal DNA in S. toebii was excluded by the results of the conventional 1% agarose gel electrophoresis and the field inversion gel electrophoresis of undigested S. toebii DNA.
Keyword
symbiobacterium toebiiobligately commensal thermophilegenome
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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