Local structural elements in the mostly unstructured transcriptional activation domain of human p53

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dc.contributor.authorHyun Lee-
dc.contributor.authorK Hun Mok-
dc.contributor.authorRanjith Muhandiram-
dc.contributor.authorKyu Hwan Park-
dc.contributor.authorJae Eun Suk-
dc.contributor.authorDo Hyung Kim-
dc.contributor.authorJun Chang-
dc.contributor.authorYoung Chul Sung-
dc.contributor.authorKwan Yong Choi-
dc.contributor.authorKyou Hoon Han-
dc.date.accessioned2017-04-19T08:57:06Z-
dc.date.available2017-04-19T08:57:06Z-
dc.date.issued2000-
dc.identifier.issn00219258-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5112-
dc.description.abstractDNA transcription is initiated by a small regulatory region of transactivators known as the transactivation domain. In contrast to the rapid progress made on the functional aspect of this promiscuous domain, its structural feature is still poorly characterized. Here, our multidimensional NMR study reveals that an unbound full-length p53 transactivation domain, although similar to the recently discovered group of loosely folded proteins in that it does not have tertiary structure, is nevertheless populated by an amphipathic helix and two nascent turns. The helix is formed by residues Thr18-Leu26 (Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu), whereas the two turns are formed by residues Met40-Met44 and Asp48-Trp53, respectively. It is remarkable that these local secondary structures are selectively formed by functionally critical and positionally conserved hydrophobic residues present in several acidic transactivation domains. This observation suggests that such local structures are general features of acidic transactivation domains and may represent 'specificity determinants' (Ptashne, M., and Gann, A. A. F. (1997), Nature 386, 569-577) that are important for transcriptional activity.-
dc.publisherAmer Soc Biochemistry Molecular Biology Inc-
dc.titleLocal structural elements in the mostly unstructured transcriptional activation domain of human p53-
dc.title.alternativeLocal structural elements in the mostly unstructured transcriptional activation domain of human p53-
dc.typeArticle-
dc.citation.titleJournal of Biological Chemistry-
dc.citation.number38-
dc.citation.endPage29432-
dc.citation.startPage29426-
dc.citation.volume275-
dc.contributor.alternativeName이현-
dc.contributor.alternativeName목헌-
dc.contributor.alternativeNameMuhandiram-
dc.contributor.alternativeName박규환-
dc.contributor.alternativeName석재은-
dc.contributor.alternativeName김도형-
dc.contributor.alternativeName장준-
dc.contributor.alternativeName성영철-
dc.contributor.alternativeName최관용-
dc.contributor.alternativeName한규훈-
dc.identifier.bibliographicCitationJournal of Biological Chemistry, vol. 275, no. 38, pp. 29426-29432-
dc.identifier.doi10.1074/jbc.M003107200-
dc.description.journalClassY-
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