Cloning of a new Bacillus thuringiensis cry1I-type crystal protein gene

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Title
Cloning of a new Bacillus thuringiensis cry1I-type crystal protein gene
Author(s)
Soo Keun Choi; Byung Sik Shin; Eun Mee Kong; Hyune Mo Rho; Seung Hwan Park
Bibliographic Citation
Current Microbiology, vol. 41, no. 1, pp. 65-69
Publication Year
2000
Abstract
A new cry1I-type gene, cry1Id1, was cloned from a B. thuringiensis isolate, and its nucleotide sequence was determined. The deduced amino acid sequence of Cry1Id1 is 89.7%, 87.2%, and 83.4% identical to the Cry1Ia, Cry1Ib, and Cry1Ic proteins, respectively. The upstream sequence of the cry1Id1 structural gene was not functional as promoter in B. subtilis. The Cry1Id1 protein, purified from recombinant E. coli cells, had a toxicity comparable to that of Cry1Ia against Plutella xylostella, but it was significantly less active than Cry1Ia against Bombyx mori. Cry1Id1 was not active against the coleopteran insect, Agelastica coerulea.
ISSN
0343-8651
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s002840010093
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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