Overexpression of an alkaline lipase gene from Proteus vulgaris K80 in Escherichia coli BL21/pKLE

Cited 8 time in scopus
Metadata Downloads
Title
Overexpression of an alkaline lipase gene from Proteus vulgaris K80 in Escherichia coli BL21/pKLE
Author(s)
Hong Weon Lee; Sun Jun Yoon; Hyung Kwon Kim; Kyong Mun Park; Tae Kwang Oh; Joon Ki Jung
Bibliographic Citation
Biotechnology Letters, vol. 22, no. 19, pp. 1543-1547
Publication Year
2000
Abstract
Over-expression of Proteus vulgaris K80 lipase gene in Escherichia coli BL21 (DE3)/pKLE was achieved, with the enzyme being produced in an as active soluble form, using the T7 RNA polymerase system in a modified M9 salt or M9ZB medium. D-Lactose (55 mM) was used to induce gene expression and gave twice the lipase activity achieved with 0.4 mM IPTG. The expression of the lipase gene depended on the feeding rate of glucose being optimal at 12 g 1-1 h-1.
Keyword
E. coli BL21/pKLElipaseoverexpression
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005621532280
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.