Effect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos

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dc.contributor.authorYong Kook Kang-
dc.contributor.authorJung Sun Park-
dc.contributor.authorChul Sang Lee-
dc.contributor.authorYoung Il Yeom-
dc.contributor.authorYong Mahn Han-
dc.contributor.authorAn Sik Chung-
dc.contributor.authorKyung Kwang Lee-
dc.date.accessioned2017-04-19T08:57:09Z-
dc.date.available2017-04-19T08:57:09Z-
dc.date.issued2000-
dc.identifier.issn1040-452X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5126-
dc.description.abstractBased on the assumption that foreign DNA sequences may have increased chance of integration into the host genome if they are flanked by high copy- numbered genomic sequences such as SINEs (short interspersed elements), we investigated the integration frequency of Lac Z reporter gene flanked by a fused B1/B2 in an in vivo system using pronuclear microinjection technique in the mouse. The SINE-flanked DNA showed a 4-fold increased integration frequency of the reporter gene than the control DNA (63% vs. 16%). Moreover, the level of β-galactosidase expression, estimated from the X-Gal staining intensity in transgenic embryos, was greatly higher in SINE-carrying DNA. These results suggest that the SINE sequences can serve a very useful tool in improving the efficiency of current transgenic animal technology.-
dc.publisherWiley-
dc.titleEffect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos-
dc.title.alternativeEffect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos-
dc.typeArticle-
dc.citation.titleMolecular Reproduction and Development-
dc.citation.number3-
dc.citation.endPage371-
dc.citation.startPage366-
dc.citation.volume56-
dc.contributor.affiliatedAuthorYong Kook Kang-
dc.contributor.affiliatedAuthorJung Sun Park-
dc.contributor.affiliatedAuthorChul Sang Lee-
dc.contributor.affiliatedAuthorYoung Il Yeom-
dc.contributor.affiliatedAuthorYong Mahn Han-
dc.contributor.affiliatedAuthorKyung Kwang Lee-
dc.contributor.alternativeName강용국-
dc.contributor.alternativeName박정선-
dc.contributor.alternativeName이철상-
dc.contributor.alternativeName염영일-
dc.contributor.alternativeName한용만-
dc.contributor.alternativeName정안식-
dc.contributor.alternativeName이경광-
dc.identifier.bibliographicCitationMolecular Reproduction and Development, vol. 56, no. 3, pp. 366-371-
dc.identifier.doi10.1002/1098-2795(200007)56:3<366::AID-MRD6>3.0.CO;2-O-
dc.subject.keywordtransgene-
dc.subject.keywordSINE-
dc.subject.keywordintegration-
dc.subject.keywordmicroinjection-
dc.subject.localtransgene-
dc.subject.localTransgenes-
dc.subject.localTransgene-
dc.subject.localSINE-
dc.subject.localIntegration-
dc.subject.localintegration-
dc.subject.localMicroinjection-
dc.subject.localmicroinjection-
dc.description.journalClassY-
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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