DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yong Kook Kang | - |
dc.contributor.author | Jung Sun Park | - |
dc.contributor.author | Chul Sang Lee | - |
dc.contributor.author | Young Il Yeom | - |
dc.contributor.author | Yong Mahn Han | - |
dc.contributor.author | An Sik Chung | - |
dc.contributor.author | Kyung Kwang Lee | - |
dc.date.accessioned | 2017-04-19T08:57:09Z | - |
dc.date.available | 2017-04-19T08:57:09Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 1040-452X | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5126 | - |
dc.description.abstract | Based on the assumption that foreign DNA sequences may have increased chance of integration into the host genome if they are flanked by high copy- numbered genomic sequences such as SINEs (short interspersed elements), we investigated the integration frequency of Lac Z reporter gene flanked by a fused B1/B2 in an in vivo system using pronuclear microinjection technique in the mouse. The SINE-flanked DNA showed a 4-fold increased integration frequency of the reporter gene than the control DNA (63% vs. 16%). Moreover, the level of β-galactosidase expression, estimated from the X-Gal staining intensity in transgenic embryos, was greatly higher in SINE-carrying DNA. These results suggest that the SINE sequences can serve a very useful tool in improving the efficiency of current transgenic animal technology. | - |
dc.publisher | Wiley | - |
dc.title | Effect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos | - |
dc.title.alternative | Effect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos | - |
dc.type | Article | - |
dc.citation.title | Molecular Reproduction and Development | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 371 | - |
dc.citation.startPage | 366 | - |
dc.citation.volume | 56 | - |
dc.contributor.affiliatedAuthor | Yong Kook Kang | - |
dc.contributor.affiliatedAuthor | Jung Sun Park | - |
dc.contributor.affiliatedAuthor | Chul Sang Lee | - |
dc.contributor.affiliatedAuthor | Young Il Yeom | - |
dc.contributor.affiliatedAuthor | Yong Mahn Han | - |
dc.contributor.affiliatedAuthor | Kyung Kwang Lee | - |
dc.contributor.alternativeName | 강용국 | - |
dc.contributor.alternativeName | 박정선 | - |
dc.contributor.alternativeName | 이철상 | - |
dc.contributor.alternativeName | 염영일 | - |
dc.contributor.alternativeName | 한용만 | - |
dc.contributor.alternativeName | 정안식 | - |
dc.contributor.alternativeName | 이경광 | - |
dc.identifier.bibliographicCitation | Molecular Reproduction and Development, vol. 56, no. 3, pp. 366-371 | - |
dc.identifier.doi | 10.1002/1098-2795(200007)56:3<366::AID-MRD6>3.0.CO;2-O | - |
dc.subject.keyword | transgene | - |
dc.subject.keyword | SINE | - |
dc.subject.keyword | integration | - |
dc.subject.keyword | microinjection | - |
dc.subject.local | transgene | - |
dc.subject.local | Transgenes | - |
dc.subject.local | Transgene | - |
dc.subject.local | SINE | - |
dc.subject.local | Integration | - |
dc.subject.local | integration | - |
dc.subject.local | Microinjection | - |
dc.subject.local | microinjection | - |
dc.description.journalClass | Y | - |
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