Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

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Title
Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat
Author(s)
Jung Ho Ko; Chul Sang Lee; Kui Hyun Kim; Myung Geol Pang; Ja Shin Koo; Nanzhu Fang; Deog Bon Koo; Keon Bong Oh; Woo Sik Youn; Guo Dong Zheng; Jung Sun Park; Sun Jung Kim; Yong Mahn Han; In Young Choi; Joon Ho Lim; Sang Tae Shin; Seung Won Jin; Kyung Gwang Lee; Ook Joon Yoo
Bibliographic Citation
Transgenic Research, vol. 9, no. 3, pp. 215-222
Publication Year
2000
Abstract
We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 μg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.
Keyword
biological activitybioreactorG-CSFO-glycosylationtransgenic goat
ISSN
0962-8819
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1008972010351
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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