Role of peroxiredoxins in regulating intracellular hydrogen peroxide and hydrogen peroxide-induced apoptosis in thyroid cells
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- Role of peroxiredoxins in regulating intracellular hydrogen peroxide and hydrogen peroxide-induced apoptosis in thyroid cells
- Ho Kim; Tae Hoon Lee; Eun Shin Park; Jae Mi Suh; Soo Jung Park; Hyo Kyun Chung; O Yu Kwon; Young Kun Kim; Heung Kyu Ro; Min Ho Shong
- Bibliographic Citation
- Journal of Biological Chemistry, vol. 275, no. 24, pp. 18266-18270
- Publication Year
- Peroxiredoxins (Prxs) play an important role in regulating cellular differentiation and proliferation in several types of mammalian cells. One mechanism for this action involves modulation of hydrogen peroxide (H2O2)- mediated cellular responses. This report examines the expression of Prx I and Prx II in thyroid cells and their roles in eliminating H2O2 produced in response to thyrotropin (TSH). Prx I and Prx II are constitutively expressed in FRTL-5 thyroid cells. Prx I expression, but not Prx H expression, is stimulated by exposure to TSH and H2O2. In addition, methimazole induces a high level of Prx I mRNA and protein in these cells. Overexpression of Prx I and Prx II enhances the elimination of H2O2 produced by TSH in FRTL-5 cells. Treatment with 500 μM H2O2 causes apoptosis in FRTL-5 cells as evidenced by standard assays of apoptosis (i.e. terminal deoxynucleotidyl transferase deoxyuridine triphosphate-biotin nick end labeling, BAX expression, and poly(ADP-ribose) polymerase cleavage. Overexpression of Plax I and Prx II reduces the amount of H2O2-induced apoptosis measured by these assays. These resuits suggest that Prx I and Prx II are involved in the removal of H2O2 in thyroid cells and can protect these cells from undergoing apoptosis. These proteins are likely to be involved in the normal physiological response to TSH-induced production of H2O2 in thyroid cells.
- Amer Soc Biochemistry Molecular Biology Inc
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