Cloning and expression of the ccpA gene encoding catabolite control protein from Thermoactinomyces sp. E79

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Title
Cloning and expression of the ccpA gene encoding catabolite control protein from Thermoactinomyces sp. E79
Author(s)
Min Woo Moon; Sun Yang Park; Hyung Kwon Kim; Do Young Yum; Hahn Shik Kwak; Tae Kwang Oh; Jung Kee Lee
Bibliographic Citation
Bioscience Biotechnology and Biochemistry, vol. 64, no. 10, pp. 2254-2258
Publication Year
2000
Abstract
The gene ccpA encoding the catabolite control protein A (CcpA) of Thermoactinomyces sp. E79 has been cloned and characterized. Nucleotide sequence analysis of the CcpA clone showed that the cloned fragment contained the full structural gene for a protein of 346 amino acids. The predicted amino acid sequence shows similarity to the transcriptional regulators of the LacI-GaIR family; a highly conserved helix-turn-helix motif, which might bind to DNA, was identified through comparison with regulator proteins in this family. The highest sequence identity was obtained when it was compared with the CcpA of Bacillus subtilis (60%) or Bacillus megaterium (60%). The expression of ccpA in Thermoactinomyces sp. E79 was dependent on glucose, which is contrast to the cases of B. subtilis, B. megaterium and S. xylosus. The complementation experiment with the B. megaterium ccpA mutant indicated that the cloned gene was a ccpA.
Keyword
catabolite control protein Acatabolite repressionthermoactinomycesalkaline protease
ISSN
0916-8451
Publisher
T&F (Taylor & Francis)
DOI
http://dx.doi.org/10.1271/bbb.64.2254
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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