Staphylococcus haemolyticus lipase: high-level expression in Escherichia coli and activation of nonionic detergent

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dc.contributor.authorByung Chul Oh-
dc.contributor.authorHyung Kwoun Kim-
dc.contributor.authorMyung Hee Kim-
dc.contributor.authorJung Kee Lee-
dc.contributor.authorTae Kwang Oh-
dc.date.accessioned2017-04-19T08:57:12Z-
dc.date.available2017-04-19T08:57:12Z-
dc.date.issued2000-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5153-
dc.description.abstractA high level of Staphylococcus haemolyticus L62 lipase was expressed in an Escherichia coli transformant. The expressed lipase activity in the cell-free extract was 70,800 U/l, which corresponded to 30% of the total cellular proteins. Pre-mixing of the L62 lipase with some nonionic detergents enhanced its hydrolytic activity towards olive oil: Tween detergents activated the L62 lipase by 3 fold. Gel filtration chromatography of the Tween-80-L62 lipase mixture demonstrated a polymerized complex (~180 kDa) formed exclusively between Tween-80 and the L62 lipase. The lipase enzyme in the complex showed a higher specific activity towards most triacylglycerols than the intact L62 lipase. The activity enhancement towards each substrate was quite different depending on the acyl chain length; the activity towards tributyrin, trilinolein, and trilinolenin was much more enhanced than that towards the medium and the long-chain saturated triglycerides.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleStaphylococcus haemolyticus lipase: high-level expression in Escherichia coli and activation of nonionic detergent-
dc.title.alternativeStaphylococcus haemolyticus lipase: high-level expression in Escherichia coli and activation of nonionic detergent-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number5-
dc.citation.endPage662-
dc.citation.startPage656-
dc.citation.volume10-
dc.contributor.affiliatedAuthorByung Chul Oh-
dc.contributor.affiliatedAuthorHyung Kwoun Kim-
dc.contributor.affiliatedAuthorJung Kee Lee-
dc.contributor.affiliatedAuthorTae Kwang Oh-
dc.contributor.alternativeName오병철-
dc.contributor.alternativeName김형권-
dc.contributor.alternativeName김명희-
dc.contributor.alternativeName이정기-
dc.contributor.alternativeName오태광-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 10, no. 5, pp. 656-662-
dc.subject.keywordLipase-
dc.subject.keywordStaphylococcus haemolyticus-
dc.subject.keywordnonionic detergent-
dc.subject.locallipase-
dc.subject.localLipase-
dc.subject.localStaphylococcus haemolyticus-
dc.subject.localnonionic detergent-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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