Synthesis of methy1 beta-D-fructoside catalyzed by levansucrase from Rahnella aquatilis

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Title
Synthesis of methy1 beta-D-fructoside catalyzed by levansucrase from Rahnella aquatilis
Author(s)
Min Gon Kim; Chul Ho Kim; Jong Sik Lee; Ki Bang Song; Sang Ki Rhee
Bibliographic Citation
Enzyme and Microbial Technology, vol. 27, no. 9, pp. 646-651
Publication Year
2000
Abstract
Methyl β-D-fructoside(MF) was formed from sucrose and methanol by a transfructosylation reaction using recombinant levansucrase from Rahnella aquatilis. The increase in the yield of MF formation was achieved by increasing methanol concentration. The enzyme stability at higher concentrations of methanol was maintained by lowering the reaction temperature. The optimum temperature and sucrose concentration for MF formation was 10°C and 50 gL-1 respectively and the yield of MF was 70%.
Keyword
Alkyl glycosideMethyl β-D-fructosidelevansucraseRahnella aquatilis
ISSN
0141-0229
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0141-0229(00)00267-2
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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