Characterization of the mouse dynamin I gene promoter and identification of sequences that direct expression in neuronal cells

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dc.contributor.authorJi Yun Yoo-
dc.contributor.authorSang Seop Lee-
dc.contributor.authorMoon Jin Jeong-
dc.contributor.authorKyung Im Lee-
dc.contributor.authorByoung-Mog Kwon-
dc.contributor.authorSung Hoon Kim-
dc.contributor.authorYoung Mee Park-
dc.contributor.authorMi Young Han-
dc.date.accessioned2017-04-19T08:57:13Z-
dc.date.available2017-04-19T08:57:13Z-
dc.date.issued2000-
dc.identifier.issn0264-6021-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5160-
dc.description.abstractDynamin I is expressed at high levels in brain and its expression is regulated during the developmental stages of brain. To elucidate the molecular mechanism by which the expression is tissue-specifically regulated, we cloned the 5'-flanking region of the mouse dynamin I gene and determined the nucleotide sequence of 1036 bases upstream from the translation start site. Transient transfection studies with a chloramphenicol acetyl-transferase reporter gene in neuroblastoma NS20Y and Lewis lung cells demonstrated that the 5'-flanking region has a cell-type-specific promoter activity. Deletion analyses demonstrated that the minimal promoter activity was detected in the proximal region 195 bp upstream of the translation initiation codon (-90 to + 105). The minimal promoter was embedded in a GC-rich region (75% GC content), in which an Sp1-binding motif and a nuclear factor (NF)-κB-like element (NE-1) were found, but it lacked TATA and CAAT boxes. Mutational analysis and electrophoretic mobility-shift assay analysis revealed that Sp1 binds to the Sp1 site and that this element is critical for the promoter activity of the dynamin I gene. We found that the NE-1 sequence is required for the expression of the dynamin I gene but NEBP (NE-1-binding protein), which binds to the NE-1 sequence, is not NF-κB. We also found that one base in the NE-1 sequence (the underlined G residue in GGGATTCGCGGA) is critical for binding specificity to discriminate between NEBP and NF-κB. By UV cross-linking analysis, we found that NEBP is an approx. 104 kDa nuclear protein.-
dc.publisherPortland Press Ltd-
dc.titleCharacterization of the mouse dynamin I gene promoter and identification of sequences that direct expression in neuronal cells-
dc.title.alternativeCharacterization of the mouse dynamin I gene promoter and identification of sequences that direct expression in neuronal cells-
dc.typeArticle-
dc.citation.titleBiochemical Journal-
dc.citation.number3-
dc.citation.endPage668-
dc.citation.startPage661-
dc.citation.volume351-
dc.contributor.affiliatedAuthorJi Yun Yoo-
dc.contributor.affiliatedAuthorSang Seop Lee-
dc.contributor.affiliatedAuthorMoon Jin Jeong-
dc.contributor.affiliatedAuthorKyung Im Lee-
dc.contributor.affiliatedAuthorByoung-Mog Kwon-
dc.contributor.affiliatedAuthorMi Young Han-
dc.contributor.alternativeName유지윤-
dc.contributor.alternativeName이상섭-
dc.contributor.alternativeName정문진-
dc.contributor.alternativeName이경임-
dc.contributor.alternativeName권병목-
dc.contributor.alternativeName김성훈-
dc.contributor.alternativeName박영미-
dc.contributor.alternativeName한미영-
dc.identifier.bibliographicCitationBiochemical Journal, vol. 351, no. 3, pp. 661-668-
dc.identifier.doi10.1042/0264-6021:3510661-
dc.subject.keyworddynamin I-
dc.subject.keywordnuclear factor κB-
dc.subject.keywordpromoter-
dc.subject.keywordSp1-
dc.subject.localdynamin I-
dc.subject.localNuclear factor-kappa B-
dc.subject.localnuclear factor κB-
dc.subject.localNf-κb-
dc.subject.localNF-kB-
dc.subject.localnuclear factor kappa B-
dc.subject.localNF-κB (nuclear factor kappa-B)-
dc.subject.localNF-kappaB-
dc.subject.localNuclear factor-κb-
dc.subject.localNF-κ B-
dc.subject.localNF-κB-
dc.subject.localNF-kappa B-
dc.subject.localNuclear factor κB (NF-κB)-
dc.subject.localNuclear factor κB-
dc.subject.localNFκB-
dc.subject.localNf-κB-
dc.subject.localNuclear factor-κB-
dc.subject.localnuclear factorκB-
dc.subject.localNuclear factor (NF)-κB-
dc.subject.localNuclear factor kappa B-
dc.subject.localnuclear factor-κB-
dc.subject.localNF-ΚB-
dc.subject.localNuclear factor-kappa B (NF-κB)-
dc.subject.localNuclear factor-kappaB-
dc.subject.localnuclear factor-kappaB-
dc.subject.localnuclear factor-kappaB (NF-κB)-
dc.subject.localNFkappaB-
dc.subject.localNuclear factor kappaB-
dc.subject.localPromoter-
dc.subject.localpromoter-
dc.subject.localPromoters-
dc.subject.localSP1-
dc.subject.localSp1-
dc.subject.localSP-1-
dc.description.journalClassY-
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