Ultrastructural and molecular identification of a wolbachia endosymbiont in a spider, nephila clavata

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Title
Ultrastructural and molecular identification of a wolbachia endosymbiont in a spider, nephila clavata
Author(s)
Hyun Woo Oh; Mi Gwang Kim; Sang Woon Shin; Kyung Sook Bae; Young Joon Ahn; Ho Yong Park
Bibliographic Citation
Insect Molecular Biology, vol. 9, no. 5, pp. 539-543
Publication Year
2000
Abstract
Wolbachia-like bacteria were observed in the egg cells of golden orb-weaving spider, Nephila clavata, by means of transmission electron microscopy. The bacteria exhibited, the typical morphology of Wolbachia, including three enveloping membranes. Based on the amplification and sequencing of partial 16S rDNA and ftsZ gene, the bacteria were identified as Wolbachia, intracellular, transovarially inherited α-proteobacteria in invertebrates. Phylogenetic analysis based on 16S rDNA and ftsZ gene sequences invariably indicated that the intracellular bacteria from N. clavata belonged to group A Wolbachia, which were found only from insects. Clustering of Wolbachia from N. clavata with group A Wolbachia indicates that the bacteria were probably transferred horizontally between insects and the spider.
Keyword
Wolbachia16S rDNAftsZNephila clavataspider
ISSN
0962-1075
Publisher
Wiley
DOI
http://dx.doi.org/10.1046/j.1365-2583.2000.00218.x
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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