Determination of the protective effects of neutralizing anti-hepatitis B virus (HBV) immunoglobulins by epitope mapping with recombinant HBV surface-antigen proteins

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dc.contributor.authorJung Hyun Park-
dc.contributor.authorEun Wie Cho-
dc.contributor.authorYun Jung Lee-
dc.contributor.authorSong Yub Shin-
dc.contributor.authorKil Lyong Kim-
dc.date.accessioned2017-04-19T08:57:14Z-
dc.date.available2017-04-19T08:57:14Z-
dc.date.issued2000-
dc.identifier.issn03855600-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5165-
dc.description.abstractAnti-hepatitis B virus (HBV) surface-antigen immunoglobulins prepared from human sera are clinical reagents which have been approved for prophylactic treatment in HBV-exposed persons. The passive immunoprophylaxis with immunoglobulins is meant to cross-link viral particles, which are then further cleared by the host's own immune system. While antibodies specific for both anti-S- and anti-preS proteins have been proved to serve as effective anti-viral agents, so far the fine antigen specificity of clinical immunoglobulin preparations has not been determined. Using recombinant proteins covering the hepatitis B surface antigen, in the present study, the specificity of a commercially available immunoglobulin preparation was determined and immunodominant epitopes were mapped. Here, it is shown that the major reactivity of anti-HBV immunoglobulins is directed against the S-protein, and that no reactivity to the preS2 but a weak binding activity to the preS1 region was detectable. The antigen reactivity within the preS1 region was biased to the C-terminal region, which indicates the presence of a putative B-cell epitope. The evaluation of the antigen specificity and determination of novel protective epitopes will provide valuable information for the further development and improvement of prophylactic HBV immunoglobulins.-
dc.publisherWiley-
dc.titleDetermination of the protective effects of neutralizing anti-hepatitis B virus (HBV) immunoglobulins by epitope mapping with recombinant HBV surface-antigen proteins-
dc.title.alternativeDetermination of the protective effects of neutralizing anti-hepatitis B virus (HBV) immunoglobulins by epitope mapping with recombinant HBV surface-antigen proteins-
dc.typeArticle-
dc.citation.titleMicrobiology and Immunology-
dc.citation.number8-
dc.citation.endPage710-
dc.citation.startPage703-
dc.citation.volume44-
dc.contributor.affiliatedAuthorEun Wie Cho-
dc.contributor.alternativeName박정현-
dc.contributor.alternativeName조은위-
dc.contributor.alternativeName이윤정-
dc.contributor.alternativeName신송엽-
dc.contributor.alternativeName김길룡-
dc.identifier.bibliographicCitationMicrobiology and Immunology, vol. 44, no. 8, pp. 703-710-
dc.identifier.doi10.1111/j.1348-0421.2000.tb02552.x-
dc.subject.keywordhepatitis B virus-
dc.subject.keywordimmunoglobulin-
dc.subject.keywordPreS-
dc.subject.keywordS-protein-
dc.subject.localhepatitis B virus-
dc.subject.localHepatitis B virus (HBV)-
dc.subject.localimmunoglobulin-
dc.subject.localPreS-
dc.subject.localS-protein-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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