Genomic organization and tissue-specific expression of rat urocortin

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dc.contributor.authorJung Hyun Park-
dc.contributor.authorYun Jung Lee-
dc.contributor.authorShin Young Na-
dc.contributor.authorKil Lyong Kim-
dc.date.accessioned2017-04-19T08:57:14Z-
dc.date.available2017-04-19T08:57:14Z-
dc.date.issued2000-
dc.identifier.issn03043940-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5167-
dc.description.abstractThe genomic organization of rat urocortin was determined using both cDNA and liver genomic DNA as templates for polymerase chain reactions. A single intron of 261 bp was found upstream to the ATG start codon, and the whole urocortin coding sequence was shown to be contained in a single exon. Relying on these results, oligonucleotide primers were designed, which can differentiate genomic DNA contamination from cDNA-derived signals in a reverse transcription-polymerase chain reaction. Tissue screening for urocortin expression revealed that the mid-brain is the major site of urocortin mRNA expression, and that other peripheral organs, including lymphoid tissues and peripheral blood lymphocytes, do not produce urocortin.-
dc.publisherElsevier-
dc.titleGenomic organization and tissue-specific expression of rat urocortin-
dc.title.alternativeGenomic organization and tissue-specific expression of rat urocortin-
dc.typeArticle-
dc.citation.titleNeuroscience Letters-
dc.citation.number1-
dc.citation.endPage48-
dc.citation.startPage45-
dc.citation.volume292-
dc.contributor.alternativeName박정현-
dc.contributor.alternativeName이윤정-
dc.contributor.alternativeName나신영-
dc.contributor.alternativeName김길룡-
dc.identifier.bibliographicCitationNeuroscience Letters, vol. 292, no. 1, pp. 45-48-
dc.identifier.doi10.1016/S0304-3940(00)01434-8-
dc.subject.keywordrat-
dc.subject.keywordurocortin-
dc.subject.keywordgenomic organization-
dc.subject.keywordintron-
dc.subject.keywordperipheral blood lymphocytes-
dc.subject.keywordreverse transcription-polymerase chain reaction-
dc.subject.localrat-
dc.subject.localRat-
dc.subject.localurocortin-
dc.subject.localUrocortin-
dc.subject.localgenomic organization-
dc.subject.localGenomic organization-
dc.subject.localintron-
dc.subject.localIntron-
dc.subject.localperipheral blood lymphocytes-
dc.subject.localreverse transcription-polymerase chain reaction-
dc.subject.localReverse transcription-polymerase chain reaction (RT-PCR)-
dc.description.journalClassY-
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