DC Field | Value | Language |
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dc.contributor.author | Young Jin Jeon | - |
dc.contributor.author | Seung H Han | - |
dc.contributor.author | Yong W Lee | - |
dc.contributor.author | Michael Lee | - |
dc.contributor.author | Kyu H Yang | - |
dc.contributor.author | Hwan Mook Kim | - |
dc.date.accessioned | 2017-04-19T08:57:15Z | - |
dc.date.available | 2017-04-19T08:57:15Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0162-3109 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5170 | - |
dc.description.abstract | In the present study, the mechanism by which dexamethasone (DEX) inhibited IL-1β gene expression in bacterial lipopolysaccharide (LPS)-activated RAW 264.7 cells was investigated. The decrease in LPS-induced IL-1β mRNA expression was demonstrated by quantitative reverse transcription polymerase chain reaction (RT-PCR). Since the promoter in IL-1β gene contains binding motifs for NF-κB/Rel, AP-1, NF-IL6, and CREB/ATF, which appear to be important in LPS-mediated IL-1β induction, the effects of DEX on the activation of these transcription factors were examined. Treatment of DEX to RAW 264.7 cells induced a dose-related inhibition of NF-κB/Rel and AP-1 in chloramphenicol acetyltransferase activity, while neither NF-IL6 nor CREB/ATF activation was affected by DEX. Treatment of RAW 264.7 cells with DEX inhibited DNA binding of NF-κB/Rel and AP-1 proteins to their cognate DNA sites as measured by electrophoretic mobility shift assay (EMSA). DEX treatment caused a significant reduction in nuclear c-rel, p65, and p50 protein contents, and these decreases were paralleled by the accumulation of cytoplasmic c-rel, p65, and p50. DEX treatment of RAW 264.7 cells did not inhibit the nuclear translocation of c-jun and c-fos. We found that the inhibition of IL-1β production by DEX is not related to p38, which is important in the IL-1β induction. These results suggest that DEX may inhibit IL-1β gene expression by a mechanism involving the blocking of LPS-induced NF-κB/Rel and AP-1 activation. | - |
dc.publisher | Elsevier | - |
dc.title | Dexamethasone inhibits IL-1β gene expression in LPS-stimulated RAW 264.7 cells by blocking NF-κB/Rel and AP-1 activation | - |
dc.title.alternative | Dexamethasone inhibits IL-1β gene expression in LPS-stimulated RAW 264.7 cells by blocking NF-κB/Rel and AP-1 activation | - |
dc.type | Article | - |
dc.citation.title | Immunopharmacology | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 183 | - |
dc.citation.startPage | 173 | - |
dc.citation.volume | 48 | - |
dc.contributor.affiliatedAuthor | Young Jin Jeon | - |
dc.contributor.affiliatedAuthor | Seung H Han | - |
dc.contributor.affiliatedAuthor | Michael Lee | - |
dc.contributor.affiliatedAuthor | Hwan Mook Kim | - |
dc.contributor.alternativeName | 전영진 | - |
dc.contributor.alternativeName | 한승현 | - |
dc.contributor.alternativeName | 이용우 | - |
dc.contributor.alternativeName | 이미가엘 | - |
dc.contributor.alternativeName | 양규환 | - |
dc.contributor.alternativeName | 김환묵 | - |
dc.identifier.bibliographicCitation | Immunopharmacology, vol. 48, no. 2, pp. 173-183 | - |
dc.identifier.doi | 10.1016/S0162-3109(00)00199-5 | - |
dc.subject.keyword | dexamethasone | - |
dc.subject.keyword | IL-1β | - |
dc.subject.keyword | NF-κB/Rel | - |
dc.subject.keyword | AP-1 | - |
dc.subject.keyword | glucocorticoid receptor | - |
dc.subject.local | dexamethasone | - |
dc.subject.local | Dexamethasone | - |
dc.subject.local | IL-1β | - |
dc.subject.local | NF-κB/Rel | - |
dc.subject.local | AP-1 | - |
dc.subject.local | glucocorticoid receptor | - |
dc.description.journalClass | Y | - |
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