Characteristics of levan fructotransferase from Arthrobacter ureafaciens K2032 and difructose anhydride IV formation from levan

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Title
Characteristics of levan fructotransferase from Arthrobacter ureafaciens K2032 and difructose anhydride IV formation from levan
Author(s)
Ki Bang Song; Kyung Sook Bae; Yong Bok Lee; Ki Young Lee; Sang Ki Rhee
Bibliographic Citation
Enzyme and Microbial Technology, vol. 27, no. 3, pp. 212-218
Publication Year
2000
Abstract
A microorganism producing levan fructotransferase was isolated from sugar-disclosed soil and it was identified as Arthrobacter ureafaciens. The major product from levan by enzyme reaction was identified as di-D-fructofuranose 2,6':6,2' dianhydride by mass spectrometry, nuclear magnetic resonance, and chemical analyses. Small amounts of several oligosaccharides and free fructose were also formed by enzyme reaction. An extracellular enzyme that produces di-D-fructofuranose 2,6':6,2' dianhydride from levan was purified from the culture broth of A. ureafaciens K2032. The enzyme had optimum activity around pH 5.8 and 45°C and had a dimeric form in solution. The N-terminal amino acid residues of the purified enzyme were SAPGSLRAVYHMTPPSGXLXDPQ. The enzyme has narrow substrate range and converts the levan to di-D-fructofuranose 2,6':6,2' dianhydride with around 62.5% conversion yield.
Keyword
arthrobacter ureafaciens K-2032levan fructotransferasepurificationDFA IV
ISSN
0141-0229
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0141-0229(00)00135-6
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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