A novel microbial interaction: obligate commensalism between a new gram-negative thermophile and a thermophilic Bacillus strain

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Title
A novel microbial interaction: obligate commensalism between a new gram-negative thermophile and a thermophilic Bacillus strain
Author(s)
Sung-Keun Rhee; Seung Goo Lee; Seung Pyo Hong; Yoon Ho Choi; Jong Hoon Park; Chul Joong Kim; Moon Hee Sung
Bibliographic Citation
Extremophiles, vol. 4, no. 3, pp. 131-136
Publication Year
2000
Abstract
Obligately commensal interaction between a new gram-negative thermophile and a thermophilic Bacillus strain was investigated. From compost samples, a mixed culture showing tyrosine phenol-lyase activity was enriched at 60°C. The mixed culture consisted of a thermophilic gram-negative strain, SC-1, and a gram-positive spore-forming strain, SK-1. In mixed cultures, strain SC-1 started to grow only when strain SK-1 entered the stationary phase. Although strain SC-1 showed tyrosine phenol lyase activity, we could not isolate a colony with any nutrient medium. For the isolation and cultivation of strain SC-1, we added culture supernatant and cell extract of the mixed culture to the basal medium. The supernatant and cell extract of the mixed culture contained heat-stable and heat-labile factors, respectively, that are essential to the growth of strain SC-1. During pure cultures of strain SK-1, the heat-stable growth factors were released during the growth phase and the heat-labile growth factors were produced intracellularly at the early stationary phase. Strain SC-1 was gram-negative and microaerophilic, and grows optimally at 60°C. Based on these results, we propose a novel commensal interaction between a new gram-negative thermophile, strain SC-1, and Bacillus sp. strain SK-1.
Keyword
CommensalismGram-negative thermophileGrowth factorsTyrosine phenollyase
ISSN
1431-0651
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s007920070027
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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