Mouse monoclonal antibodies to hepatitis B virus preS1 produced after immunization with recombinant preS1 peptide

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dc.contributor.authorChun Jeih Ryu-
dc.contributor.authorYoun Kyu Kim-
dc.contributor.authorHyang Suk Hur-
dc.contributor.authorHee Sun Kim-
dc.contributor.authorJai Min Oh-
dc.contributor.authorYoung Jun Kang-
dc.contributor.authorHyo Jeong Hong-
dc.date.accessioned2017-04-19T08:57:16Z-
dc.date.available2017-04-19T08:57:16Z-
dc.date.issued2000-
dc.identifier.issn0272-457X-
dc.identifier.uri10.1089/02724570050031248ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5178-
dc.description.abstractWe have efficiently generated mouse monoclonal antibodies (MAbs), which bind specifically to amino acids 21-47 of the preS1 domain of hepatitis B virus (HBV) by immunizing mice with the preS1 peptide (amino acids, aa 1-56) conjugated to keyhole limpet hemocyanin. Hybridomas were screened by an indirect enzyme-linked immunosorbent assay (ELISA) using the purified preS1 peptide as a coated antigen. Eighteen positive hybridomas were selected and subjected to isotyping. of these, 5 clones secreted immunoglobulin G (IgG) and 13 clones secreted IgM. Four (KR1, KR2, KR3, and KR4) of the 5 IgG MAbs bound to preS1 peptide (aa 21-47). Epitope mapping using bacterially expressed GST fusion proteins revealed that three clones (KR2, KR3, KR4) (IgG1, κ) recognize aa 21-35, while KR1 (IgG2a, κ) recognizes aa 35-47 of the preS1. These MAbs immunoprecipitated HBV particles, demonstrating that they bind to native HBV particles.-
dc.publisherMary Ann Liebertko
dc.titleMouse monoclonal antibodies to hepatitis B virus preS1 produced after immunization with recombinant preS1 peptide-
dc.title.alternativeMouse monoclonal antibodies to hepatitis B virus preS1 produced after immunization with recombinant preS1 peptide-
dc.typeArticle-
dc.citation.titleHybridoma-
dc.citation.number2-
dc.citation.endPage189-
dc.citation.startPage185-
dc.citation.volume19-
dc.contributor.affiliatedAuthorChun Jeih Ryu-
dc.contributor.affiliatedAuthorYoun Kyu Kim-
dc.contributor.affiliatedAuthorHyang Suk Hur-
dc.contributor.affiliatedAuthorYoung Jun Kang-
dc.contributor.affiliatedAuthorHyo Jeong Hong-
dc.contributor.alternativeName류춘제-
dc.contributor.alternativeName김윤규-
dc.contributor.alternativeName허향숙-
dc.contributor.alternativeName김희선-
dc.contributor.alternativeName오재민-
dc.contributor.alternativeName강영준-
dc.contributor.alternativeName홍효정-
dc.identifier.bibliographicCitationHybridoma, vol. 19, no. 2, pp. 185-189-
dc.identifier.doi10.1089/02724570050031248-
dc.description.journalClassN-
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