Involvement of SH2-SH2-SH3 domain of phospholipase cγ1 in NF-κB signaling

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dc.contributor.authorBo Yeon Kim-
dc.contributor.authorDae Ook Kang-
dc.contributor.authorWon Keun Oh-
dc.contributor.authorJin Hee Kim-
dc.contributor.authorYong Kyung Choe-
dc.contributor.authorJong Soo Jang-
dc.contributor.authorPann Gill Suh-
dc.contributor.authorSung Ho Ryu-
dc.contributor.authorTae Ick Mheen-
dc.contributor.authorJong Seog Ahn-
dc.date.accessioned2017-04-19T08:57:17Z-
dc.date.available2017-04-19T08:57:17Z-
dc.date.issued2000-
dc.identifier.issn0014-5793-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5186-
dc.description.abstractTo directly define the role of phospholipase Cγ1 (PLCγ1) in NF-κB activation, NF-κB promoted luciferase reporter gene plasmid (pNF-κB-Luc) was transfected into rat-3Y1 fibroblasts that overexpress whole PLCγ1 (PLCγ1-3Y1), src homology domains SH2-SH2-SH3 of PLCγ1 (SH223-3Y1) and v-src (Src-3Y1). Transient transfection with pNF-κB-Luc remarkably increased the luciferase activity in all three transformants compared with normal rat-3Y1 cells. Pretreatment with inhibitors of protein tyrosine kinase reduced this increase in luciferase activity, but U73122 (a PLC inhibitor) did not. While PD98059, an inhibitor of mitogen activated protein kinase (MAPK), significantly reduced the luciferase activity, there was no effect by wortmannin and Ro-31-8220, inhibitors of phosphatidylinositol 3-kinase (PI3K) and protein kinase C (PKC), respectively. This study shows a direct evidence that the SH2-SH2-SH3 region of PLCγ1 contributes to the NF-κB signaling and that MAPK, but not PI3K and PKC, is involved in SH2-SH2-SH3 mediated NF-κB activation in these cells.-
dc.publisherWiley-
dc.titleInvolvement of SH2-SH2-SH3 domain of phospholipase cγ1 in NF-κB signaling-
dc.title.alternativeInvolvement of SH2-SH2-SH3 domain of phospholipase cγ1 in NF-κB signaling-
dc.typeArticle-
dc.citation.titleFEBS Letters-
dc.citation.number1-
dc.citation.endPage49-
dc.citation.startPage45-
dc.citation.volume472-
dc.contributor.affiliatedAuthorBo Yeon Kim-
dc.contributor.affiliatedAuthorDae Ook Kang-
dc.contributor.affiliatedAuthorWon Keun Oh-
dc.contributor.affiliatedAuthorJin Hee Kim-
dc.contributor.affiliatedAuthorYong Kyung Choe-
dc.contributor.affiliatedAuthorTae Ick Mheen-
dc.contributor.affiliatedAuthorJong Seog Ahn-
dc.contributor.alternativeName김보연-
dc.contributor.alternativeName강대욱-
dc.contributor.alternativeName오원근-
dc.contributor.alternativeName김진희-
dc.contributor.alternativeName최용경-
dc.contributor.alternativeName장종수-
dc.contributor.alternativeName서판길-
dc.contributor.alternativeName류성호-
dc.contributor.alternativeName민태익-
dc.contributor.alternativeName안종석-
dc.identifier.bibliographicCitationFEBS Letters, vol. 472, no. 1, pp. 45-49-
dc.identifier.doi10.1016/S0014-5793(00)01415-0-
dc.subject.keywordMitogen activated protein kinase-
dc.subject.keywordNuclear factor κB-
dc.subject.keywordPhosphatidylinositol 3-kinase-
dc.subject.keywordPhospholipase Cγ1-
dc.subject.keywordProtein kinase C-
dc.subject.keywordSH2-SH3-
dc.subject.localmitogen-activated protein kinase-
dc.subject.localMitogen activated protein kinase-
dc.subject.localMitogen-activated protein (MAP) kinase-
dc.subject.localmitogen-activated protein kinases-
dc.subject.localMitogen-activated protein kinase (MAPK)-
dc.subject.localMitogen-activated protein kinase-
dc.subject.localMitogenactivated protein kinase-
dc.subject.localmitogen activated protein kinase-
dc.subject.localMitogen-activated protein kinases (MAPKs)-
dc.subject.localMitogen-acti-vated protein kinase-
dc.subject.localMitogen-activated protein kinases-
dc.subject.localNuclear factor-kappa B-
dc.subject.localnuclear factor κB-
dc.subject.localNf-κb-
dc.subject.localNF-kB-
dc.subject.localnuclear factor kappa B-
dc.subject.localNF-κB (nuclear factor kappa-B)-
dc.subject.localNF-kappaB-
dc.subject.localNuclear factor-κb-
dc.subject.localNF-κ B-
dc.subject.localNF-κB-
dc.subject.localNF-kappa B-
dc.subject.localNuclear factor κB (NF-κB)-
dc.subject.localNuclear factor κB-
dc.subject.localNFκB-
dc.subject.localNf-κB-
dc.subject.localNuclear factor-κB-
dc.subject.localnuclear factorκB-
dc.subject.localNuclear factor (NF)-κB-
dc.subject.localNuclear factor kappa B-
dc.subject.localnuclear factor-κB-
dc.subject.localNF-ΚB-
dc.subject.localNuclear factor-kappa B (NF-κB)-
dc.subject.localNuclear factor-kappaB-
dc.subject.localnuclear factor-kappaB-
dc.subject.localnuclear factor-kappaB (NF-κB)-
dc.subject.localNFkappaB-
dc.subject.localNuclear factor kappaB-
dc.subject.localPhosphatidylinositol 3-kinase-
dc.subject.localPhospholipase Cγ1-
dc.subject.localphospholipase Cγ1-
dc.subject.localphospholipase C-γ1-
dc.subject.localProtein kinase C-
dc.subject.localprotein kinase C-
dc.subject.localprotein kinase c-
dc.subject.localSH2-SH3-
dc.description.journalClassY-
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