Presence of Multiple cDNAs Encoding an Isoform of ADP-Glucose Pyrophosphorylase Large Subunit from Sweet Potato and Characterization of Expression Levels

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dc.contributor.authorChee Hark Harn-
dc.contributor.authorJung Myung Bae-
dc.contributor.authorSang Sook Lee-
dc.contributor.authorSung Ran Min-
dc.contributor.authorJang Ryol Liu-
dc.description.abstractThree cDNAs (iAGPLI-1, iAGPLI-2, and iAGPLI-3) encoding an isoform of AGPase large subunit were isolated from a sweet potato cDNA library constructed from tuberous root tissue. iAGPLI-1 was 2,161 bp in length and contained an open reading frame of 517 amino acids with a calculated molecular mass of 57,689 Da. iAGPLI-2 and iAGPLI-3 were 1,804 and 1,524 bp in length, respectively, and contained partial open reading frames of 490 and 385 amino acids. Deduced amino acid sequence comparison analysis showed that iAGPLI-1 has sequence identity with iAGPLI-2 (97.9) and iAGPLI-3 (98.7%) while iAGPLI-2 and iAGPLI-3 have 96.8% sequence identity, iAGPLI-1 had the highest sequence identity of 77.8% with potato AGPase (sAGPL1). Steady-state levels of iAGPLI-1 transcripts were expressed predominantly in the stem, and moderately in the tuberous root, but not in either the roots or leaves. However, AGPase activity was present in all tissues. The expression level in the stem declined dramatically after a 12 h incubation in the dark to nearly 3% of the value under light, although the activity under a dark condition remained at half the levels in light. The activity levels were not correlated with the transcript levels. iAGPL transcripts in leaves were induced by sucrose feeding but not by glucose or fructose. Therefore, the expression of iAGPLI-1 is regulated in stem tissue preferentially and by sucrose. Southern blot analysis showed that the sweet potato genome contained several copies of iAGPLI gene probably due to polyploidy.-
dc.publisherOxford Univ Press-
dc.titlePresence of Multiple cDNAs Encoding an Isoform of ADP-Glucose Pyrophosphorylase Large Subunit from Sweet Potato and Characterization of Expression Levels-
dc.title.alternativePresence of Multiple cDNAs Encoding an Isoform of ADP-Glucose Pyrophosphorylase Large Subunit from Sweet Potato and Characterization of Expression Levels-
dc.citation.titlePlant and Cell Physiology-
dc.contributor.affiliatedAuthorJung Myung Bae-
dc.contributor.affiliatedAuthorSang Sook Lee-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.identifier.bibliographicCitationPlant and Cell Physiology, vol. 41, no. 11, pp. 1235-1242-
dc.subject.keywordADP-glucose pyrophosphorylase large subunit-
dc.subject.keywordAGPase activity-
dc.subject.keywordsweet potato-
dc.subject.keywordstarch biosynthesis-
dc.subject.localADP-glucose pyrophosphorylase large subunit-
dc.subject.localAGPase activity-
dc.subject.localsweet potato-
dc.subject.localsweet potatoes-
dc.subject.localSweet potato (Ipomoea batatas L. Lam)-
dc.subject.localSweet potato (Ipomoea batatas)-
dc.subject.localSweet otato-
dc.subject.localsweet potato (Ipomoea batatas)-
dc.subject.localipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas)-
dc.subject.localSweetpotato Ipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas L.)-
dc.subject.localSweetpotato (Ipomoea batatas (L.) Lam)-
dc.subject.localSweet potato-
dc.subject.localIpomoea batatas-
dc.subject.localSweet potato (Ipomoea batatas (L.) Lam.)-
dc.subject.localstarch biosynthesis-
dc.subject.localStarch biosynthesis-
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Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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