Detection of cellular receptors specific for the hepatitis B virus preS surface protein on cell lines of extrahepatic origin

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dc.contributor.authorJung Hyun Park-
dc.contributor.authorEun A Choi-
dc.contributor.authorEun Wie Cho-
dc.contributor.authorYun Jung Lee-
dc.contributor.authorJung Min Park-
dc.contributor.authorShin Young Na-
dc.contributor.authorKil Lyong Kim-
dc.date.accessioned2017-04-19T08:57:18Z-
dc.date.available2017-04-19T08:57:18Z-
dc.date.issued2000-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5194-
dc.description.abstractHepatitis B virus infection is primarily mediated by the interaction of the preS region of the viral envelope protein with its still unknown cellular receptor. Using recombinantly expressed preS proteins, the distribution of preS-binding receptors on cell lines from extra-hepatic origins was determined by immunofluorescence and flow cytometry. In contrast to human liver cell lines, most cell lines from extrahepatic origins did not bind preS proteins. Nevertheless, exceptions were found in the bone marrow, derived cell line, KG-1, and the osteogenic sarcoma cell line SaOS-2, as well as in the previously reported EBV-transformed B-cell line, Wa. To determine the biochemical nature of these receptors, Wa-cells were cell surface biotinylated and the preS-binding receptors were isolated by immunoprecipitation. A specific band with a molecular weight of -39 kDa was identified in a SDS-polyacrylamide gel, which further characterization is expected to provide clues regarding the infection mechanism of HBV in hepatic- and extra-hepatic cells.-
dc.publisherElsevier-
dc.titleDetection of cellular receptors specific for the hepatitis B virus preS surface protein on cell lines of extrahepatic origin-
dc.title.alternativeDetection of cellular receptors specific for the hepatitis B virus preS surface protein on cell lines of extrahepatic origin-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number1-
dc.citation.endPage254-
dc.citation.startPage246-
dc.citation.volume277-
dc.contributor.affiliatedAuthorJung Hyun Park-
dc.contributor.affiliatedAuthorEun A Choi-
dc.contributor.affiliatedAuthorEun Wie Cho-
dc.contributor.affiliatedAuthorYun Jung Lee-
dc.contributor.affiliatedAuthorJung Min Park-
dc.contributor.affiliatedAuthorShin Young Na-
dc.contributor.affiliatedAuthorKil Lyong Kim-
dc.contributor.alternativeName박정현-
dc.contributor.alternativeName최은아-
dc.contributor.alternativeName조은위-
dc.contributor.alternativeName이윤정-
dc.contributor.alternativeName박정민-
dc.contributor.alternativeName나신영-
dc.contributor.alternativeName김길룡-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 277, no. 1, pp. 246-254-
dc.identifier.doi10.1006/bbrc.2000.3661-
dc.subject.keywordhepatitis B virus-
dc.subject.keywordpreS protein-
dc.subject.keywordextrahepatic cells-
dc.subject.keywordreceptor-
dc.subject.keywordflow cytometry-
dc.subject.localhepatitis B virus (HBV)-
dc.subject.localHepatitis B Virus-
dc.subject.localHepatitis B virus (HBV)-
dc.subject.localhepatitis B virus-
dc.subject.localhepatitis B Virus (HBV)-
dc.subject.localHepatitis B virus-
dc.subject.localpreS protein-
dc.subject.localextrahepatic cells-
dc.subject.localReceptors-
dc.subject.localreceptor-
dc.subject.localReceptor-
dc.subject.localFlow cytometry-
dc.subject.localflow cytometry-
dc.description.journalClassY-
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Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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