Statistical optimization of seed and induction conditions to enhance phytase production by recombinant Escherichia coli

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Title
Statistical optimization of seed and induction conditions to enhance phytase production by recombinant Escherichia coli
Author(s)
K Sunitha; Young Ok Kim; Jung Kee Lee; Tae Kwang Oh
Bibliographic Citation
Biochemical Engineering Journal, vol. 5, no. 1, pp. 51-56
Publication Year
2000
Abstract
An efficient fermentation process for the production of phytase was developed by statistical optimization from recombinant Escherichia coli BL21. The seed age and inoculum level highly influenced the expression of phytase gene by lactose induction. The optimum of 3.15-h seed culture age instead of an overnight culture and 4.8% (v/v) seed culture yielded high concentrations of cells at short intervals of time for efficient induction. Induction at a lactose concentration of 7.28 mM and at a cell optical density of 0.55 at 550 nm (equivalent to 0.23 g/l dry cell weight) was found to be most suitable in this system. The time of induction and lactose concentration significantly influenced phytase production. The optimized conditions were used to achieve 100% increase in phytase production (174 U/l · h) compared to production before optimizing the conditions.
Keyword
phytasestatistical optimizationfermentationlactoseenzyme production
ISSN
1369-703X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S1369-703X(99)00062-5
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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