Statistical optimization of seed and induction conditions to enhance phytase production by recombinant Escherichia coli

Abstract

An efficient fermentation process for the production of phytase was developed by statistical optimization from recombinant Escherichia coli BL21. The seed age and inoculum level highly influenced the expression of phytase gene by lactose induction. The optimum of 3.15-h seed culture age instead of an overnight culture and 4.8% (v/v) seed culture yielded high concentrations of cells at short intervals of time for efficient induction. Induction at a lactose concentration of 7.28 mM and at a cell optical density of 0.55 at 550 nm (equivalent to 0.23 g/l dry cell weight) was found to be most suitable in this system. The time of induction and lactose concentration significantly influenced phytase production. The optimized conditions were used to achieve 100% increase in phytase production (174 U/l · h) compared to production before optimizing the conditions.