Synthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2]

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dc.contributor.authorVibhor Saraswat-
dc.contributor.authorJee Won Lee-
dc.contributor.authorDae Young Kim-
dc.contributor.authorYoung Hoon Park-
dc.date.accessioned2017-04-19T08:57:18Z-
dc.date.available2017-04-19T08:57:18Z-
dc.date.issued2000-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5197-
dc.description.abstractUsing lactose as an inducer, recombinant human interleukin-2 (rhIL-2) was synthesized with an N-terminus fusion partner, G3 (three tandem-arranged glucagon peptides) in fed-batch cultures at high cell concentration (60-90 g l-1) of Escherichia coli BL21(DE3) [pT7-G3IL2]. With batch additions of lactose (4 x 13.5 g), the fusion rhIL-2 was synthesized up to 9.3 g l-1. However, if all the lactose (54 g) was added at once to the culture, synthesized fusion rhIL-2 decreased to 5.4 g l-1 with a decreased cell growth rate. A statistical optimization of the production medium containing glucose, yeast extract, and lactose led to fusion rhIL-2 being produced at > 9 g l-1.-
dc.publisherSpringer-
dc.titleSynthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2]-
dc.title.alternativeSynthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2]-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number4-
dc.citation.endPage265-
dc.citation.startPage261-
dc.citation.volume22-
dc.contributor.affiliatedAuthorJee Won Lee-
dc.contributor.affiliatedAuthorDae Young Kim-
dc.contributor.affiliatedAuthorYoung Hoon Park-
dc.contributor.alternativeNameSaraswat-
dc.contributor.alternativeName이지원-
dc.contributor.alternativeName김대영-
dc.contributor.alternativeName박영훈-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 22, no. 4, pp. 261-265-
dc.identifier.doi10.1023/A:1005638602146-
dc.subject.keywordEscherichia coli-
dc.subject.keywordInterleukin-2-
dc.subject.keywordlactose-
dc.subject.keywordsymport-
dc.subject.localEscherichia coli.-
dc.subject.localescherichia coli-
dc.subject.localEscherichia Coli-
dc.subject.localEscherichia coli-
dc.subject.localE.coli-
dc.subject.localescherichia coil-
dc.subject.localE. coli-
dc.subject.localE. Coli-
dc.subject.localinterleukin-2-
dc.subject.localInterleukin-2-
dc.subject.localLactose-
dc.subject.locallactose-
dc.subject.localsymport-
dc.description.journalClassY-
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