Regulation of starch contents in potato (Solanum tuberosum L.) by manipulation of sucrose synthase gene

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Title
Regulation of starch contents in potato (Solanum tuberosum L.) by manipulation of sucrose synthase gene
Author(s)
Hyun Soon KimJae Heung Jeon; Kyung Hwa Choi; Young Hee Joung; Byoung Il Lee; Hyouk Joung
Bibliographic Citation
Journal of Japanese Society for Horticultural Science, vol. 69, no. 3, pp. 243-249
Publication Year
2000
Abstract
Transgenic potato microtubers were created in which the expression of sucrose synthase (Susy) was altered by introducing a chimeric gene containing the coding region of Susy that is linked to the patatin promoter in sense (S) or antisense (AS) orientation. The analysis of transgenic microtubers showed that Susy activity and starch content increased in S plants 8, 22, and 30 transformed with the chimeric sense gene; they decreased in AS plants 3 and 10 transformed with the antisense gene. RT-PCR analysis revealed that the level of mRNA for Susy increased in S plants, and decreased in AS plants. There was a little change in the expression of genes for ADP-glucose pyrophosphorylase (AGPase), one of key enzymes in starch biosynthesis. The level of mRNA for AGPase and the activity of the enzyme increased in S 8 plant, but decreased in AS 3 plant.
Keyword
microtuberpatatin promoterstarch contentsucrose synthasetransgenic potato
ISSN
0013-7626
DOI
http://dx.doi.org/10.2503/jjshs.69.243
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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