Anti-apoptotic effects of terrein on etoposide-induced apoptosis of U937 human leukemia cells = Terrein의 etoposide에 의해 유도된 apoptosis 저해효과

Abstract

In the course of screening for the substances inhibiting apoptosis of U937 human leukemia cell induced by etoposide, a fungal strain F80834 producing a high level of inhibitor was selected. The inhibitory substance was purified and identified as terrein by spectroscopic methods of UV, EI-MS, 1H-NMR, 13C-NMR and DEPT. Terrein showed inhibitory activity of caspase 3, a major protease of apoptosis cascade, with an IC50 value of 20 μg/ml after 7 hrs of treatment. It also showed protective effect against cell death with an IC50 value of 10 μg/ml on U937 cells induced by etoposide after 24 hrs of treatment, but did not show any cytotoxicity at the same condition without etoposide.