Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower

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Title
Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower
Author(s)
Suk Weon Kim; Jang Ryol Liu
Bibliographic Citation
Plant Cell Tissue and Organ Culture, vol. 58, no. 3, pp. 227-230
Publication Year
1999
Abstract
Mature zygotic embryos of balloon flower (Platycodon grandiflorum) formed embryogenic calluses at a frequency of 43% when cultured on Murashige and Skoog medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Cell suspension cultures were established from embryogenic calluses using MS liquid medium with 4.52 μM 2,4-D. Following transfer to solid MS basal medium, cell suspension cultures gave rise to somatic embryos, which then developed into plantlets. Plantlets were transplanted to potting soil and grown to maturity.
Keyword
campanulaceaeplatycodon grandiflorumsomatic embryosuspension culture
ISSN
0167-6857
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1006338024745
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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