Enhancement of extracellular glucose oxidase production in pH-stat feed-back controlled fed-batch culture of recombinant Saccharomyces cerevisiae

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Title
Enhancement of extracellular glucose oxidase production in pH-stat feed-back controlled fed-batch culture of recombinant Saccharomyces cerevisiae
Author(s)
Arnab Kapat; Joon Ki Jung; Young Hoon Park
Bibliographic Citation
Biotechnology Letters, vol. 20, no. 7, pp. 683-686
Publication Year
1998
Abstract
Glucose oxidase (GOD) from Aspergillus niger was expressed in Saccharomyces cerevisiae under the regime of GAL-10 promoter and GAL-7 terminator of S. cerevisiae and α-amylase signal sequence of Aspergillus oryzae. The enhancement of the expression level was achieved in pH-stat feed-back controlled fed-batch culture. The highest titre of extracellular GOD was 199 U/ml which marked two fold improvement over the batch (95 U/ml) and 28% above that of non-feed back controlled fed-batch (154 U/ml) operation.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005374608270
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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