Cloning and sequence analysis of another Shiga-like toxin IIe variant gene (slt-IIera) from an Escherichia coli R107 strain isolated from rabbit

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Title
Cloning and sequence analysis of another Shiga-like toxin IIe variant gene (slt-IIera) from an Escherichia coli R107 strain isolated from rabbit
Author(s)
Sang Hyun Kim; In Ho Cha; Kyoung Sook Kim; Yong Hwan Kim; Young Choon Lee
Bibliographic Citation
Microbiology and Immunology, vol. 41, no. 10, pp. 805-808
Publication Year
1997
Abstract
An Escherichia coli R107 strain (O26 serotype) producing a Shiga-like toxin IIe variant (SLT-IIera) was isolated fram the mesenteric lymph node of a freshly dead rabbit carcass. The entire structural gene for this SLT-IIera was cloned from chromosomal DNA by PCR using primers based on previously published slt-IIe sequences. Nucleotide sequence analysis indicated that the slt-IIera gene was very similar to slt-IIe (formerly called slt-IIv) from E. coli strains S1191 and 412; five and one nucleotide changes were detected in A and B subunits, respectively, which resulted in changes in amino acid sequences of the corresponding subunits by three and one residues. Recombinant SLT-IIera and SLT-IIe produced using an E. coli host-vector system showed similar cytotoxicity, suggesting that the variations in the structural gene of SLT-IIera have no significant effect on cytotoxic level.
Keyword
Shiga toxin genePathogenesis
ISSN
0385-5600
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1348-0421.1997.tb01931.x
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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