Effect of continuous feeding of galactose on the production of recombinant glucose oxidase using Saccharomyces cerevisiae

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Title
Effect of continuous feeding of galactose on the production of recombinant glucose oxidase using Saccharomyces cerevisiae
Author(s)
Arnab Kapat; Joon Ki Jung; Young Hoon Park
Bibliographic Citation
Bioprocess Engineering, vol. 23, no. 1, pp. 37-40
Publication Year
2000
Abstract
A recombinant strain of Saccharomyces cerevisiae harboring GOD gene originated from Aspergillus niger was used for the production of extracellular glucose oxidase. The effect of continuous galactose feeding on the induction of GAL-10 promoter was examined in a 5 l bioreactor. The highest enzyme production level (164 U cm-3) was achieved at 96 h of cultivation. The production performance was compared with the results of fed-batch cultivations carried out in the same laboratory. Continuous feeding mode was found to be less productive due to excess ethanol formation and plasmid instability.
Keyword
galactoseglucose oxidaserecombinant enzymeSaccharomyces cerevisiae
ISSN
0178-515X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s004499900117
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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