Analysis of production of thermostable alkaline protease using thermoactinomyces sp. E79 = Thermoactinomyces sp. E79를 이용한 내열성 Alkaline 단백질 분해효소 생산 : 환경인자의 영향

Cited 0 time in scopus
Metadata Downloads
Title
Analysis of production of thermostable alkaline protease using thermoactinomyces sp. E79 = Thermoactinomyces sp. E79를 이용한 내열성 Alkaline 단백질 분해효소 생산 : 환경인자의 영향
Author(s)
Sang Won Jung; Sung Sik Park; Yong Cheol Park; Tae Kwang Oh; Jin Ho Seo
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 28, no. 3, pp. 167-171
Publication Year
2000
Abstract
This research was undertaken to analyze fermentation properties of Thermoactinomyces sp. E79 for production of a thermostable alkaline protease, which is able to specifically hydrolyze defatted soybean meal (DSM) to amino acids. The optimum pH for cell growth and protease production was pH 6.7. Thermoactinomyces sp. E79 did not grow at pH10 Among carbon sources tested, soluble starch was the best for protease production, while glucose repressed protease production. Tryptone was found to be the best nitrogen source for cell growth and soytone was good for protease production. Oxygen transfer rate played an important role in producing thermostable alkaline protease. Maximum values of 6.58 g/L of dry cell weight and 43.0 U/mL of protease activity were obtained in a batch fermentation using a 2.5 L jar fermentor at 1.93 x 102 hr-1 of volumetric oxygen transfer coefficient (κ(L)a). Addition of 200 mg/L humic acid to the growth medium resulted in 1.64 times higher protease activity and 1.77 times higher cell growth than the case without humic acid addition.
Keyword
Thermoactinomyces sp.Thermostable alkaline protease
ISSN
0257-2389
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.