Time dependency of the action of nitric oxide in lipopolysaccharide-interferon-γ-induced neuronal cell death in murine primary neuron-glia co-cultures

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Title
Time dependency of the action of nitric oxide in lipopolysaccharide-interferon-γ-induced neuronal cell death in murine primary neuron-glia co-cultures
Author(s)
G H Jeohn; Won Gon Kim; J S Hong
Bibliographic Citation
Brain Research, vol. 880, no. 1, pp. 173-177
Publication Year
2000
Abstract
We investigated the time-dependency of the action of nitric oxide (NO) on glia-mediated neuronal cell death. Cortical neuron-glia co-cultures were treated with lipopolysaccharide and interferon γ (LPS/IFNγ). The production of NO was first detectable 9 h after the exposure to LPS/IFNγ and increased for up to 48 h. A significant neuronal cell death was observed 36-48 h after treatment with LPS/IFNγ. The NO generated at the initial stage of NO synthesis (about 12 h) following exposure to LPS/IFNγ was found to be critical for LPS/IFNγ-induced neurotoxicity. Furthermore, the rate of NO production at the initial stage of NO synthesis was correlated linearly with the extent of neuronal cell death. These findings suggest that the maximal rate of NO synthesis, instead of the accumulated NO2- level, is a sensitive index for predicting endotoxin-induced cytotoxicity.
Keyword
nitric oxidelipopolysaccharideneuronsglianeurotoxicityprimary cell culturecytokines
ISSN
0006-8993
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0006-8993(00)02737-2
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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