Developmental patterns of mST3GalV mRNA expression in the mouse: in situ hybridization using DIG-labeled RNA probes

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Title
Developmental patterns of mST3GalV mRNA expression in the mouse: in situ hybridization using DIG-labeled RNA probes
Author(s)
Min Young Ji; Young Choon Lee; Su Il Do; Sang Yun Nam; Kyu Yong Jung; Hyoung Min Kim; Jong Kun Park; Young Kug Choo
Bibliographic Citation
Archives of Pharmacal Research, vol. 23, no. 5, pp. 525-530
Publication Year
2000
Abstract
mSTSCalV synthesizes ganglioside GM3, the precursor for simple and complex a- and b- series gangliosides, and the expression and regulation of mSTSGalV (CMP-NeuAc: lactosylceramide α2,3-sialyltransferase) activity is central to the production of almost all gangliosides, a class of glycosphingolipids implicated in variety of cellular processes such as transmembrane signaling, synaptic transmission, specialized membrane domain formation and cell-cell interactions. To understand the developmental expression of mST3CalV in mice, we investigated the spatial and temporal expression of mSTBGalV mRNA during the mouse embryogenesis [embryonic (E) days; E9, E11, E13, E15] by in situ hybridization with digoxigenin-labeled RNA probes. All tissues from E9 and E11 were positive for mST3GalV mRNA. On E13, mST3GalV mRNA was expressed in various neural and non-neural tissues. In contrast to these, on E15, the telencephalon and liver produced a strong expression of mST3Gal V which was a quite similar to that of E13. In this stage, mST3GalV mRNA was also expressed in some non-neural tissues. These data indicate that mST3GalV is differently expressed at developmental stages of embryo, and this may be importantly related with regulation of organogenesis in mice.
Keyword
mST3GalVembryogenesis
ISSN
0253-6269
Publisher
Pharmaceutical Soc Korea
DOI
http://dx.doi.org/10.1007/BF02976584
Type
Article
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1. Journal Articles > Journal Articles
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