Molecular identification of Wolbachia naturally infected in Thecodiplosis japonensis(Diptera: cecidominideii)

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Title
Molecular identification of Wolbachia naturally infected in Thecodiplosis japonensis(Diptera: cecidominideii)
Author(s)
Mi Gwang Kim; Hyun Woo Oh; Hee Moon Park; Ho Yong Park
Bibliographic Citation
Korean Journal of Entomology, vol. 30, no. 3, pp. 139-146
Publication Year
2000
Abstract
The presence and phylogenetic position of endosymbiotic bacteria were studied from the pine needle gall midge, Thecodiplosis japonensis that is the most serious insect pest of the red pine, Pinus densif lora. PCR amplification using the general primers for eubacterial16S ribosomal DNA (rDNA) and the sequence of the amplified product showed that T. japonensis harbours the obligate intracellular bacteria Wolbachia, which has been known to infect an extremely wide range of insect species, in which it induces reproductive alterations. Phylogenetic analysis of Wolbachia strain of T. japonensis based on the sequences of 16S rDNA and ftsZ gene show agreement, indicating that this bacterium belongs to A-subdivision of Wolbachia.
Keyword
thecodiplosis japonensiswolbachia16S rDNAftsZ gene
ISSN
1011-9493
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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