Inhibitory effect of coumarins on nitric oxide production in LPS-activated murine macrophages = 쿠마린에 의한 raw 264.7 세포주의 nitric oxide 생성 저해활성

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Title
Inhibitory effect of coumarins on nitric oxide production in LPS-activated murine macrophages = 쿠마린에 의한 raw 264.7 세포주의 nitric oxide 생성 저해활성
Author(s)
Tae Cheol Rho; Hee Cheol Choi; Bo Yeon Kim; Young Ho Kim; Jong Seog Ahn; Young Kook Kim; Hyun Sun Lee
Bibliographic Citation
Korean Journal of Pharmacognosy, vol. 30, no. 4, pp. 413-416
Publication Year
1999
Abstract
During the screening for inhibitors on nitric oxide (NO) production in LPS-activated murine macrophage, RAW 264.7 cells, two coumarins were isolated from chloroform extract of Ponciri Fructus. They were identified as imperatorin (10), phellopterin (11) on the basis of spectroscopic methods. The IC50 values for NO formatiom were about 5.4 μM and 35.0 μM, respectively, and then eleven coumarins were tested for the inhibitory effects on NO production in activated macrophages. All the test coumarins inhibited NO production in concentration-dependent manner and furanocoumarins (6-11) showed much more potent inhibitory effect than simple coumatins. Among the compounds examined xanthotoxin (8) was the most potent inhibitors of NO production (IC50=1.4 μM). Analysis of the structure-activity relationship among these coumarins led to the conclusion that the substitution of C-5 position in furanocoumarins reduces greatly their inhibitory potency although the substitution of C-8 does not almost affect it.
Keyword
Ponciri Fructuscoumarinsnitric oxideRAW 264.7 cellslipopolysaccharide (LPS)
ISSN
0253-3073
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
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