High level expression of human lipocortin(annexin) 1 in Escherichia coli

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dc.contributor.authorKwang Lae Hoe-
dc.contributor.authorEun Hee Cho-
dc.contributor.authorSoo Ok Lee-
dc.contributor.authorDoe Sun Na-
dc.date.accessioned2017-04-19T08:57:24Z-
dc.date.available2017-04-19T08:57:24Z-
dc.date.issued1996-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5241-
dc.description.abstractHuman lipocortin (annexin) 1, a member of the annexin family of phospholipid binding proteins, has previously been expressed in E. coli (Huh et al., 1990). To improve the expression level of lipocortin 1 in E. coli, several expression vectors containing either the PL or the Ptrc promoter were constructed. The highest expression level, up to 20% of the total E. coli proteins, was obtained with plasmid pHT3. Plasmid pHT3 contains the pUC origin, the lipocortin 1 cDNA under Ptrc promoter, and the locI gene.-
dc.publisherSpringer-
dc.titleHigh level expression of human lipocortin(annexin) 1 in Escherichia coli-
dc.title.alternativeHigh level expression of human lipocortin(annexin) 1 in Escherichia coli-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number2-
dc.citation.endPage168-
dc.citation.startPage163-
dc.citation.volume18-
dc.contributor.affiliatedAuthorKwang Lae Hoe-
dc.contributor.alternativeName허광래-
dc.contributor.alternativeName조은희-
dc.contributor.alternativeName이수옥-
dc.contributor.alternativeName나도선-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 18, no. 2, pp. 163-168-
dc.identifier.doi10.1007/BF00128673-
dc.subject.keywordlipocortin 1-
dc.subject.keywordescherichia coli-
dc.subject.keywordexpression vector-
dc.subject.locallipocortin 1-
dc.subject.locallipocortin-1-
dc.subject.localEscherichia coli.-
dc.subject.localescherichia coli-
dc.subject.localEscherichia Coli-
dc.subject.localEscherichia coli-
dc.subject.localE.coli-
dc.subject.localescherichia coil-
dc.subject.localE. coli-
dc.subject.localE. Coli-
dc.subject.localExpression Vector-
dc.subject.localexpression vector-
dc.subject.localExpression vector-
dc.subject.localExpression vectors-
dc.description.journalClassY-
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