High level expression of human lipocortin(annexin) 1 in Escherichia coli

Abstract

Human lipocortin (annexin) 1, a member of the annexin family of phospholipid binding proteins, has previously been expressed in E. coli (Huh et al., 1990). To improve the expression level of lipocortin 1 in E. coli, several expression vectors containing either the PL or the Ptrc promoter were constructed. The highest expression level, up to 20% of the total E. coli proteins, was obtained with plasmid pHT3. Plasmid pHT3 contains the pUC origin, the lipocortin 1 cDNA under Ptrc promoter, and the locI gene.