The Role of RhoA in the Germinal Vesicle Breakdown of Mouse Oocytes

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dc.contributor.authorYong Pil Cheon-
dc.contributor.authorSung Woo Kim-
dc.contributor.authorSoo Jung Kim-
dc.contributor.authorYoung Il Yeom-
dc.contributor.authorChae Joon Cheong-
dc.contributor.authorKwon Soo Ha-
dc.date.accessioned2017-04-19T08:57:24Z-
dc.date.available2017-04-19T08:57:24Z-
dc.date.issued2000-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5242-
dc.description.abstractWe have investigated a new role of RhoA in the germinal vesicle breakdown (GVBD) of mouse oocytes. First, RhoA was identified by immunostaining and ADP-ribosylation in germinal vesicle (GV) stage-oocytes. RhoA was mainly localized in the ooplasmic area, but rarely detected in germinal vesicle. Incubation of oocyte extract with C3 transferase induced a strong ADP-ribosylation at about 25 kDa. Incubation of GV-stage oocytes in culture medium induced the spontaneous maturation to GVBD by about 78 and 87% of total oocytes at 1 and 3 h, respectively. However, microinjection of C3 transferase into GV-stage oocytes significantly inhibited GVBD at 1 (GVBD = 29%) and 3 h (GVBD = 49%). To study the role of reactive oxygen species (ROS) in the oocyte maturation, the level of intra-oocyte ROS was measured using a ROS-specific fluorescent dye H2DCFDA during the oocyte maturation. Spontaneous maturation of GV-stage oocytes induced a significant increase of ROS at 3 h by about twofold over the control level and then the increased level was maintained until 6 h. However, microinjection of C3 transferase inhibited the production of intra-oocyte ROS. Incubation with ROS scavengers, N-acetyl-L-cysteine and catalase, blocked the ROS increase. The ROS scavengers also significantly inhibited GVBD, as did C3 transferase. Thus, it was proposed that RhoA was involved in the GVBD, possibly by the production of ROS in mouse oocytes.-
dc.publisherElsevier-
dc.titleThe Role of RhoA in the Germinal Vesicle Breakdown of Mouse Oocytes-
dc.title.alternativeThe Role of RhoA in the Germinal Vesicle Breakdown of Mouse Oocytes-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number3-
dc.citation.endPage1002-
dc.citation.startPage997-
dc.citation.volume273-
dc.contributor.affiliatedAuthorYoung Il Yeom-
dc.contributor.alternativeName천용필-
dc.contributor.alternativeName김성우-
dc.contributor.alternativeName김수정-
dc.contributor.alternativeName염영일-
dc.contributor.alternativeName정재준-
dc.contributor.alternativeName하권수-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 273, no. 3, pp. 997-1002-
dc.identifier.doi10.1006/bbrc.2000.3052-
dc.subject.keywordmouse oocyte-
dc.subject.keywordRhoA-
dc.subject.keywordreactive oxygen species-
dc.subject.keywordC3 transferase-
dc.subject.keywordGVBD-
dc.subject.localmouse oocyte-
dc.subject.localRHOA-
dc.subject.localRhoA-
dc.subject.localReactive oxidative species-
dc.subject.localReactive oxygen species(ROS)-
dc.subject.localReactive oxygen species-
dc.subject.localReactive Oxygen Species (ROS)-
dc.subject.localReactive Oxygen Species-
dc.subject.localROS-
dc.subject.localReactive oxygen species (ROS)-
dc.subject.localreactive oxygen species-
dc.subject.localreactive oxygen species (ROS)-
dc.subject.localC3 transferase-
dc.subject.localGVBD-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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