Mutagenesis of the glucoamylase signal peptide of Saccharomyces diastaticus and functional analysis in Saccharomyces cerevisiae

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Title
Mutagenesis of the glucoamylase signal peptide of Saccharomyces diastaticus and functional analysis in Saccharomyces cerevisiae
Author(s)
Jun Won Lee; Dae Ook Kang; Bo Yeon Kim; Won Keun Oh; Tae Ick Mheen; Yu Ryang Pyun; Jong Seog Ahn
Bibliographic Citation
FEMS Microbiology Letters, vol. 193, no. 1, pp. 7-11
Publication Year
2000
Abstract
To improve the efficiency of the glucoamylase signal peptide (GSP) of Saccharomyces diastaticus for the secretion of foreign proteins, hybrid plasmids containing one of four types of GSP mutant (m1, Pro-18→Leu-18; m2, Tyr-13→Leu-13; m3, Ser-9→Leu-9; m4, Asn-5→Pro-5) were constructed and evaluated in Saccharomyces cerevisiae using Bacillus endo-1,4-β-D-glucanase (CMCase) as a reporter gene. CMCase secretion by m1, m2 and m3 GSP mutants was increased, likely resulting from a higher probability of the modified GSP to assume an α-helical structure. Especially in the case of m3, the substitution of Leu for a polar residue, Ser-9, in the hydrophobic region resulted in approximately a twofold increase in extracellular CMCase activity. In mutant 4, which disrupts the α-helix of GSP, CMCase was less efficiently secreted.
Keyword
glucoamylase signal peptideSaccharomyces diastaticusBacillus endo-1,4-β-D-glucanaseMutagenesisSaccharomyces cerevisiaesecretion
ISSN
0378-1097
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1111/j.1574-6968.2000.tb09394.x
Type
Article
Appears in Collections:
Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
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