Enhancement of adenoviral transduction with polycationic liposomes in vivo

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Title
Enhancement of adenoviral transduction with polycationic liposomes in vivo
Author(s)
Sang Goo Lee; Seong Jun Yoon; Chun Dong Kim; Kyung Jin Kim; Dong Soo Im; Young Il Yeom; Myung Whun Sung; Dae Seog Heo; Noe Kyeong Kim
Bibliographic Citation
Cancer Gene Therapy, vol. 7, no. 10, pp. 1329-1335
Publication Year
2000
Abstract
Although the high transfection efficiency with adenovirus in vitro is well documented, it is still not clear whether adenoviral vectors are effective in vivo in solid tumor models. In our preliminary experiment, transduction of tumor tissue was limited to just around the injection site after intratumoral injection of the adenoviral vector. To improve the transduction efficiency in vivo, we tried a combination of adenoviral vector and liposome in our animal model. Adenovirus carrying human placental alkaline phosphatase (AdALP) and Lipofectamine or 1,3-di-oleoyloxy-2-(6-carboxyspermyl)-propylamide were used as a marker gene and the cationic liposome, respectively. A > 15-fold increase in the transfection efficiency was observed in CT26 tumor cell lines with the combination of AdALP adenovirus carrying murine granulocyte-macrophage colony-stimulating factor (AdmGM-CSF), and liposome compared with adenovirus alone, showing the feasibility of the combination treatment. In the animal model, with the combination of liposome and AdALP, deeper and wider distribution of the marker gene in the tumor mass was shown. We conclude that the limitations of direct application of adenoviral vectors in a solid tumor model could be overcome by the use of cationic liposomes. This approach will facilitate the more effective delivery of adenoviral vectors in a clinical trial setting.
Keyword
gene therapyadenovirusliposomein vivo
ISSN
0929-1903
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/sj.cgt.7700237
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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