Sequence analysis of β-lactoglobulin promoter in Korean cattle = 한우의 β-lactoglobulin 유전자 발현조절부위의 염기서열 분석

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dc.contributor.authorB C Sang-
dc.contributor.authorS H Ryoo-
dc.contributor.authorS H Lee-
dc.contributor.authorS L Yoo-
dc.contributor.authorTae Hoon Lee-
dc.contributor.authorDae Yeul Yu-
dc.date.accessioned2017-04-19T08:57:26Z-
dc.date.available2017-04-19T08:57:26Z-
dc.date.issued2000-
dc.identifier.issn03675807-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5257-
dc.description.abstractThis study was analyzed by PCR technique with specific primers in order to investigate the characterizations of β-lactoglobulin promoter in Korean cattle. This study confirmed amplified product of 776bp fragments obtained from the amplification of β-lactoglobulin promoter from genomic DNA using PCR in Korean cattle. The nucleotide sequence of β-lactoglobulin promoter in Korean cattle as compared with bovine β-lactoglobulin(β-LG) (Lorraine et al., 1997) was different in eight nucleotides and showed high homology as about 98.9%. Also, the transcriptional factors of β-lactoglobulin promoter in Korean cattle could be confirmed that existed at the same position of bovine β-lactoglobulin promoter by reported study such as AP-2(activator protein-2), NF-1(nuclear factor-1), MGF(mammary gland factor) and MPBF(milk protein binding factor). But we confirmed that Korean cattle as different from Holstein, substituted C→T at -362bp of NF-1 and -204bp of MPBF. As a consequence, The sequences of β-LG promotor was showed a high homology between Korean cattle and Holstein. Furthermore, we should be studied that relationships between the control of gene expression and nucleotide sequences of transcription factor NF-1 and MPBF in Korean cattle and Holstein. (Key words : Korean cattle, β-lactoglobulin promotor, PCR, Transcription factors)-
dc.publisherSouth Korea-
dc.titleSequence analysis of β-lactoglobulin promoter in Korean cattle = 한우의 β-lactoglobulin 유전자 발현조절부위의 염기서열 분석-
dc.title.alternativeSequence analysis of β-lactoglobulin promoter in Korean cattle-
dc.typeArticle-
dc.citation.titleJournal of Animal Science and Technology-
dc.citation.number4-
dc.citation.endPage450-
dc.citation.startPage443-
dc.citation.volume42-
dc.contributor.alternativeName상병찬-
dc.contributor.alternativeName류승희-
dc.contributor.alternativeName이상훈-
dc.contributor.alternativeName유성란-
dc.contributor.alternativeName이태훈-
dc.contributor.alternativeName유대열-
dc.identifier.bibliographicCitationJournal of Animal Science and Technology, vol. 42, no. 4, pp. 443-450-
dc.subject.keywordKorean cattle-
dc.subject.keywordβ-lactoglobulin promotor-
dc.subject.keywordPCR-
dc.subject.keywordtranscription factors-
dc.subject.localKorean cattle-
dc.subject.localKorean Cattle-
dc.subject.localβ-lactoglobulin promotor-
dc.subject.localPCR-
dc.subject.localtranscription factors-
dc.subject.localtranscription factor-
dc.description.journalClassY-
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