Cloning and in vitro translation of a human calmodulin 2 cDNA

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Cloning and in vitro translation of a human calmodulin 2 cDNA
Nam Soon Kim; Seishi Kato
Bibliographic Citation
Genes & Genomics, vol. 22, no. 3, pp. 241-247
Publication Year
During construction of a human full-length cDNA bank, we have cloned a cDNA encoding calmodulin from the human lymphoma U937 cDNA library. A database search suggests that this cDNA encodes full-length calmodulin 2 that has longest 5′ noncoding region. To examine the Ca2+-dependent binding ability of calmodulin encoded from the cDNA, it was in vitro translated in a rabbit reticulocyte lysate containing [35S] methionine and its product was mixed with 3′, 5′-cyclic nucleotide phosphodiesterase. Electrophoretic analysis on a native polyacrylamide gel revealed that the in vitro translated product of this cDNA can bind to 3′, 5′-cyclic nucleotide phosphodiesterase in the Ca2+-dependent manner. This result indicates that the in vitro translation product of the calmodulin cDNA, [35S] labeled calomodulin, can be used as a probe to assess the calmodulin-binding ability of a target protein or to screen the calmodulin binding proteins.
calmodulincalmodulin-binding proteinscDNA sequencehumanin vitro translation
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Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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