Effects of agitation and aeration on the production of extracellular glucose oxidase from a recombinant Saccharomyces cerevisiae

Cited 21 time in scopus
Metadata Downloads
Title
Effects of agitation and aeration on the production of extracellular glucose oxidase from a recombinant Saccharomyces cerevisiae
Author(s)
Arnab Kapat; Joon Ki Jung; Young Hoon Park; Sung Yong Hong; Hee Kyung Choi
Bibliographic Citation
Bioprocess Engineering, vol. 18, no. 5, pp. 347-351
Publication Year
1998
Abstract
A recombinant strain of Saccharomyces cerevisiae, GAL-GO2, was developed to facilitate the production of extracellular glucose oxidase (GOD). The recombinant strain secreted 85% (8.7 U/ml) of the total GOD (10.3 U/ml) produced in shake flask culture. For further enhancement of GOD production, optimization of the speed of agitation and the rate of aeration in a stirred tank fermentor was carried out. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular GOD was achieved when the speed of agitation and the rate of aeration were 420 rpm and 0.25 vvm, respectively. The enzyme production was increased by 74% compared to the level obtained under unoptimized conditions.
ISSN
0178-515X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s004490050454
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.