Isolation and purification of methyl mercaptan oxidase from thiobacillus thiooxidans for detection of mercaptans = 머갑탄류 검출을 위한 Thiobacillus thiooxidans가 생산하는 메칠머갑탄 산화효소의 분리 및 정제

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dc.contributor.authorSang Joon Kim-
dc.contributor.authorHyun Jae Shin-
dc.contributor.authorDae Sil Lee-
dc.contributor.authorJi Won Yang-
dc.date.accessioned2017-04-19T08:57:29Z-
dc.date.available2017-04-19T08:57:29Z-
dc.date.issued2000-
dc.identifier.issn1225-7117-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5268-
dc.description.abstractMethyl mercaptan oxidase was isolated and purified from Thiobacillus thiooxidans KCTC2505 for the detection of mercaptans. The procedure of purification involved DEAE-Sephacel and Superose 12 column chromatographies with recovery yields of 40 and 6.3%, and specific activity of 19.7 and 80.1 units/mg-protein, respectively. The molecular weight of purified methyl mercaptan oxidase was determined to be 68.1 kDa by SDS-PAGE. The extract from DEAE-Sephacel column chromatography had a high activity in oxidizing methyl mercaptan to produce formaldehyde which can be easily detected by purpald-coloring method. Optimum temperature for activity was observed at 43 degrees. This enzyme was activated by NH4CI and (NH4)2HO4, and inhibited by KCI and NaCI.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleIsolation and purification of methyl mercaptan oxidase from thiobacillus thiooxidans for detection of mercaptans = 머갑탄류 검출을 위한 Thiobacillus thiooxidans가 생산하는 메칠머갑탄 산화효소의 분리 및 정제-
dc.title.alternativeIsolation and purification of methyl mercaptan oxidase from thiobacillus thiooxidans for detection of mercaptans-
dc.typeArticle-
dc.citation.titleKorean Journal of Biotechnology and Bioengineering-
dc.citation.number2-
dc.citation.endPage149-
dc.citation.startPage145-
dc.citation.volume15-
dc.contributor.affiliatedAuthorHyun Jae Shin-
dc.contributor.affiliatedAuthorDae Sil Lee-
dc.contributor.alternativeName김상준-
dc.contributor.alternativeName신현재-
dc.contributor.alternativeName이대실-
dc.contributor.alternativeName양지원-
dc.identifier.bibliographicCitationKorean Journal of Biotechnology and Bioengineering, vol. 15, no. 2, pp. 145-149-
dc.subject.keywordmethyl mercaptan oxidase-
dc.subject.keywordthiobacillus thiooxidans-
dc.subject.keywordpurpald-
dc.subject.keywordenzyme purification-
dc.subject.localmethyl mercaptan oxidase-
dc.subject.localthiobacillus thiooxidans-
dc.subject.localpurpald-
dc.subject.localenzyme purification-
dc.description.journalClassN-
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