Isolation and purification of methyl mercaptan oxidase from thiobacillus thiooxidans for detection of mercaptans = 머갑탄류 검출을 위한 Thiobacillus thiooxidans가 생산하는 메칠머갑탄 산화효소의 분리 및 정제

Abstract

Methyl mercaptan oxidase was isolated and purified from Thiobacillus thiooxidans KCTC2505 for the detection of mercaptans. The procedure of purification involved DEAE-Sephacel and Superose 12 column chromatographies with recovery yields of 40 and 6.3%, and specific activity of 19.7 and 80.1 units/mg-protein, respectively. The molecular weight of purified methyl mercaptan oxidase was determined to be 68.1 kDa by SDS-PAGE. The extract from DEAE-Sephacel column chromatography had a high activity in oxidizing methyl mercaptan to produce formaldehyde which can be easily detected by purpald-coloring method. Optimum temperature for activity was observed at 43 degrees. This enzyme was activated by NH4CI and (NH4)2HO4, and inhibited by KCI and NaCI.