Simple purification of Escherichia coli-derived recombinant human interleukin-2 expressed with N-terminus fusion of glucagon

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dc.contributor.authorHye Soon Won-
dc.contributor.authorJee Won Lee-
dc.contributor.authorIn Ho Kim-
dc.contributor.authorYoung Hoon Park-
dc.date.accessioned2017-04-19T08:57:29Z-
dc.date.available2017-04-19T08:57:29Z-
dc.date.issued2000-
dc.identifier.issn1226-8372-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5270-
dc.description.abstractSimple procedures have been devised for purifying recombinant human interleukin-2 (hIL-2), which was expressed in Escherichia coli using sequences of glucagon molecules and enterokinase cleavage site as an N-terminus fusion partner. The insoluble aggregates of recombinant fusion protein produced in E. coli cytoplasm were easily dissolved by simple alkaline pH shift (8→12→8). Following enterokinase cleavage, the recombinant hIL-2 was finally purified by one-step reversed-phase HPLC with high purity. The ease and high efficiency of this simple purification process seem to mainly result from the role of used glucagon fusion partner, which could be applied to the production of other therapeutically important proteins.-
dc.publisherSpringer-
dc.titleSimple purification of Escherichia coli-derived recombinant human interleukin-2 expressed with N-terminus fusion of glucagon-
dc.title.alternativeSimple purification of Escherichia coli-derived recombinant human interleukin-2 expressed with N-terminus fusion of glucagon-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number1-
dc.citation.endPage16-
dc.citation.startPage13-
dc.citation.volume5-
dc.contributor.affiliatedAuthorJee Won Lee-
dc.contributor.affiliatedAuthorYoung Hoon Park-
dc.contributor.alternativeName원혜순-
dc.contributor.alternativeName이지원-
dc.contributor.alternativeName김인호-
dc.contributor.alternativeName박영훈-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 5, no. 1, pp. 13-16-
dc.identifier.doi10.1007/BF02932346-
dc.subject.keywordhuman interleukin-2-
dc.subject.keywordglucagon-
dc.subject.keywordN-terminus fusion-
dc.subject.keywordpurification process-
dc.subject.localhuman interleukin-2 (IL-2)-
dc.subject.localhuman interleukin-2-
dc.subject.localHuman interleukin-2-
dc.subject.localGlucagon-
dc.subject.localglucagon-
dc.subject.localN-terminus fusion-
dc.subject.localpurification process-
dc.description.journalClassY-
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