Improvement of gene transfer to cervical cancer cell lines using non-viral agents

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dc.contributor.authorCheong Weon Cho-
dc.contributor.authorYoung Sik Cho-
dc.contributor.authorByung Tae Kang-
dc.contributor.authorJi Sook Hwang-
dc.contributor.authorSue Nie Park-
dc.contributor.authorDo Young Yoon-
dc.date.accessioned2017-04-19T08:57:31Z-
dc.date.available2017-04-19T08:57:31Z-
dc.date.issued2001-
dc.identifier.issn0304-3835-
dc.identifier.uri10.1016/S0304-3835(00)00629-7ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5286-
dc.description.abstractVirus-like particles (VLPs) composed of recombinant capsid protein L1 and L2 of human papillomavirus type 16 were conjugated with polylysine (PL) and gene transfer was performed using VLP-PL conjugates to allow the expression of targeted gene. When HeLa cells were incubated with VLP-PL conjugate coupled with plasmid cytomegalovirus β-galactosidase (pCMVβ-gal), about 10% of cells were transfected and demonstrated β-galactosidase activity. Hence chloramphenicol acetyltransferase activity was also expressed significantly in VLP-PL-plasmid simian virus 2 chloramphenicol acetyl transferase (pSV2CAT)-transfected cells, VLP-PL conjugate was tested whether it could transfer a tumor suppressor gene, pCMVp53, to HeLa cells and the exogenously provided p53 gene complexed to VLP-PL conjugate was detected from HeLa cells by polymerase chain reaction (PCR) analysis. Interestingly, additional increase of transfection efficiency was demonstrated in the presence of poloxamer 407 when C-33A cells were transfected with VLP-PL-pCMVβ-gal complex. The result support the notion that VLP-PL conjugate may be a promising vector to transfer genetic materials into cancer cells and poloxamer 407 can be used for enhancing the transfection efficiency of VLP-PL conjugate.-
dc.publisherElsevier-
dc.titleImprovement of gene transfer to cervical cancer cell lines using non-viral agents-
dc.title.alternativeImprovement of gene transfer to cervical cancer cell lines using non-viral agents-
dc.typeArticle-
dc.citation.titleCancer Letters-
dc.citation.number1-
dc.citation.endPage85-
dc.citation.startPage75-
dc.citation.volume162-
dc.contributor.affiliatedAuthorYoung Sik Cho-
dc.contributor.affiliatedAuthorByung Tae Kang-
dc.contributor.affiliatedAuthorJi Sook Hwang-
dc.contributor.affiliatedAuthorSue Nie Park-
dc.contributor.affiliatedAuthorDo Young Yoon-
dc.contributor.alternativeName조청원-
dc.contributor.alternativeName조영식-
dc.contributor.alternativeName강병태-
dc.contributor.alternativeName황지숙-
dc.contributor.alternativeName박순희-
dc.contributor.alternativeName윤도영-
dc.identifier.bibliographicCitationCancer Letters, vol. 162, no. 1, pp. 75-85-
dc.identifier.doi10.1016/S0304-3835(00)00629-7-
dc.subject.keywordgene delivery-
dc.subject.keywordvirus-like particle-
dc.subject.keywordpolylysine-
dc.subject.keywordpoloxamer 407-
dc.subject.localgene delivery-
dc.subject.localGene delivery-
dc.subject.localvirus-like praticle-
dc.subject.localvirus-like particles-
dc.subject.localVirus-like particle-
dc.subject.localvirus-like particle-
dc.subject.localpolylysine-
dc.subject.localPoloxamer 407-
dc.subject.localpoloxamer 407-
dc.description.journalClassY-
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