DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cheong Weon Cho | - |
dc.contributor.author | Young Sik Cho | - |
dc.contributor.author | Byung Tae Kang | - |
dc.contributor.author | Ji Sook Hwang | - |
dc.contributor.author | Sue Nie Park | - |
dc.contributor.author | Do Young Yoon | - |
dc.date.accessioned | 2017-04-19T08:57:31Z | - |
dc.date.available | 2017-04-19T08:57:31Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0304-3835 | - |
dc.identifier.uri | 10.1016/S0304-3835(00)00629-7 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5286 | - |
dc.description.abstract | Virus-like particles (VLPs) composed of recombinant capsid protein L1 and L2 of human papillomavirus type 16 were conjugated with polylysine (PL) and gene transfer was performed using VLP-PL conjugates to allow the expression of targeted gene. When HeLa cells were incubated with VLP-PL conjugate coupled with plasmid cytomegalovirus β-galactosidase (pCMVβ-gal), about 10% of cells were transfected and demonstrated β-galactosidase activity. Hence chloramphenicol acetyltransferase activity was also expressed significantly in VLP-PL-plasmid simian virus 2 chloramphenicol acetyl transferase (pSV2CAT)-transfected cells, VLP-PL conjugate was tested whether it could transfer a tumor suppressor gene, pCMVp53, to HeLa cells and the exogenously provided p53 gene complexed to VLP-PL conjugate was detected from HeLa cells by polymerase chain reaction (PCR) analysis. Interestingly, additional increase of transfection efficiency was demonstrated in the presence of poloxamer 407 when C-33A cells were transfected with VLP-PL-pCMVβ-gal complex. The result support the notion that VLP-PL conjugate may be a promising vector to transfer genetic materials into cancer cells and poloxamer 407 can be used for enhancing the transfection efficiency of VLP-PL conjugate. | - |
dc.publisher | Elsevier | - |
dc.title | Improvement of gene transfer to cervical cancer cell lines using non-viral agents | - |
dc.title.alternative | Improvement of gene transfer to cervical cancer cell lines using non-viral agents | - |
dc.type | Article | - |
dc.citation.title | Cancer Letters | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 85 | - |
dc.citation.startPage | 75 | - |
dc.citation.volume | 162 | - |
dc.contributor.affiliatedAuthor | Young Sik Cho | - |
dc.contributor.affiliatedAuthor | Byung Tae Kang | - |
dc.contributor.affiliatedAuthor | Ji Sook Hwang | - |
dc.contributor.affiliatedAuthor | Sue Nie Park | - |
dc.contributor.affiliatedAuthor | Do Young Yoon | - |
dc.contributor.alternativeName | 조청원 | - |
dc.contributor.alternativeName | 조영식 | - |
dc.contributor.alternativeName | 강병태 | - |
dc.contributor.alternativeName | 황지숙 | - |
dc.contributor.alternativeName | 박순희 | - |
dc.contributor.alternativeName | 윤도영 | - |
dc.identifier.bibliographicCitation | Cancer Letters, vol. 162, no. 1, pp. 75-85 | - |
dc.identifier.doi | 10.1016/S0304-3835(00)00629-7 | - |
dc.subject.keyword | gene delivery | - |
dc.subject.keyword | virus-like particle | - |
dc.subject.keyword | polylysine | - |
dc.subject.keyword | poloxamer 407 | - |
dc.subject.local | gene delivery | - |
dc.subject.local | Gene delivery | - |
dc.subject.local | virus-like praticle | - |
dc.subject.local | virus-like particles | - |
dc.subject.local | Virus-like particle | - |
dc.subject.local | virus-like particle | - |
dc.subject.local | polylysine | - |
dc.subject.local | Poloxamer 407 | - |
dc.subject.local | poloxamer 407 | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.